Generation of melanocytes from induced pluripotent stem cells

Ruifeng Yang, Min Jiang, Suresh M. Kumar, Theodore Xu, Fei Wang, Leihong Xiang, Xiaowei Xu

Research output: Contribution to journalArticle

Abstract

Epidermal melanocytes have an important role in protecting skin from UV rays, and are implicated in a variety of skin diseases. Here, we developed an efficient method for differentiating induced pluripotent stem cells (iPSCs) into melanocytes. We first generated iPSCs from adult mouse tail-tip fibroblasts (TTFs) using retroviral vectors or virus-free piggyBac transposon vectors carrying murine Sox2, Oct3/4, c-Myc, and Klf4. The TTF-derived iPSC clones exhibited similar morphology and growth properties as mouse embryonic stem (ES) cells. The iPSCs expressed ES cell markers, displayed characteristic epigenetic changes, and formed teratomas with all three germ layers. The iPSCs were used to generate embryonic bodies and were then successfully differentiated into melanocytes by treatment with growth factors. The iPSC-derived melanocytes expressed characteristic melanocyte markers and produced melanin pigment. Electron microscopy showed that the melanocytes contained mature melanosomes. We manipulated the conditions used to differentiate iPSCs to melanocytes and discovered that Wnt3a is not required for mouse melanocyte differentiation. This report shows that melanocytes can be readily generated from iPSCs, providing a powerful resource for the in vitro study of melanocyte developmental biology and diseases. By inducing iPSCs without viruses, the possibility of integration mutagenesis is alleviated, and these iPSCs are more compatible for cell replacement therapies.

Original languageEnglish (US)
Pages (from-to)2458-2466
Number of pages9
JournalJournal of Investigative Dermatology
Volume131
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Induced Pluripotent Stem Cells
Melanocytes
Stem cells
Fibroblasts
Tail
Viruses
Skin
Virus Integration
Melanosomes
Germ Layers
Developmental Biology
Melanins
Teratoma
Embryonic Stem Cells
Mutagenesis
Cell- and Tissue-Based Therapy
Skin Diseases
Epigenomics
Intercellular Signaling Peptides and Proteins
Electron Microscopy

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Yang, R., Jiang, M., Kumar, S. M., Xu, T., Wang, F., Xiang, L., & Xu, X. (2011). Generation of melanocytes from induced pluripotent stem cells. Journal of Investigative Dermatology, 131(12), 2458-2466. https://doi.org/10.1038/jid.2011.242

Generation of melanocytes from induced pluripotent stem cells. / Yang, Ruifeng; Jiang, Min; Kumar, Suresh M.; Xu, Theodore; Wang, Fei; Xiang, Leihong; Xu, Xiaowei.

In: Journal of Investigative Dermatology, Vol. 131, No. 12, 12.2011, p. 2458-2466.

Research output: Contribution to journalArticle

Yang, R, Jiang, M, Kumar, SM, Xu, T, Wang, F, Xiang, L & Xu, X 2011, 'Generation of melanocytes from induced pluripotent stem cells', Journal of Investigative Dermatology, vol. 131, no. 12, pp. 2458-2466. https://doi.org/10.1038/jid.2011.242
Yang, Ruifeng ; Jiang, Min ; Kumar, Suresh M. ; Xu, Theodore ; Wang, Fei ; Xiang, Leihong ; Xu, Xiaowei. / Generation of melanocytes from induced pluripotent stem cells. In: Journal of Investigative Dermatology. 2011 ; Vol. 131, No. 12. pp. 2458-2466.
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