Generation of low phytic acid Arabidopsis seeds expressing an E. coli phytase during embryo development

Patricia Coello, Jeffrey P. Maughan, Alex Mendoza, Reena Philip, David W. Bollinger, Trygve L. Veum, Lila O. Vodkin, Joe C. Polacco

Research output: Contribution to journalArticlepeer-review

Abstract

An Escherichia coli phytase gene was introduced into Arabidopsis plants using an embryo-specific promoter and a signal peptide for vacuolar targeting. Three independent transgenic lines were analysed. Phytase activity in dry seeds was observed in transgenic lines, whereas no activity was detected in control, untransformed seeds. Transgenic seeds expressing the phytase gene had lower levels of phytic acid than the controls. Concomitant with the decrease in phytic acid was an increase in free phosphate. These results indicated that embryo-expressed phytase can reduce the levels of phytic acid stored during development.

Original languageEnglish (US)
Pages (from-to)285-291
Number of pages7
JournalSeed Science Research
Volume11
Issue number4
StatePublished - 2001

Keywords

  • Arabidopsis
  • Escherichia coli
  • Phytase
  • Phytic acid

ASJC Scopus subject areas

  • Plant Science

Fingerprint

Dive into the research topics of 'Generation of low phytic acid Arabidopsis seeds expressing an E. coli phytase during embryo development'. Together they form a unique fingerprint.

Cite this