Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse

Chan Jin Park, Guanglin Chen, Yongbum Koo, Po Ching P. Lin, Joseph A. Cacioppo, Hailey Prohaska, Che Myong J. Ko

Research output: Contribution to journalArticle

Abstract

Two estrogen receptors, ESR1 and ESR2, are responsible for the classical actions of estrogens in mammalian species. They display different spatiotemporal expression patterns and nonoverlapping functions in various tissues and physiological conditions. In this study, a novel knock-in mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the natural Esr1 promoter (Esr1–iCre) was developed. Functional characterization of iCre expression by crossing them with reporter lines (ROSA26-lacZ or Ai9-RFP) showed that iCre is faithfully expressed in Esr1-lineage cells. This novel transgenic mouse line will be a useful animal model for lineage-tracing Esr1-expressing cells, selective gene ablation in the Esr1-lineage cells and for generating global Esr1 knockout mice.

Original languageEnglish (US)
Article numbere23084
JournalGenesis
Volume55
Issue number12
DOIs
StatePublished - Dec 2017

Keywords

  • BAC clone
  • estrogen receptor alpha
  • iCre recombinase
  • transgenic

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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    Park, C. J., Chen, G., Koo, Y., Lin, P. C. P., Cacioppo, J. A., Prohaska, H., & Ko, C. M. J. (2017). Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse. Genesis, 55(12), [e23084]. https://doi.org/10.1002/dvg.23084