Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse

Chan Jin Park; Guanglin Chen; Yongbum Koo; Po Ching P. Lin; Joseph A. Cacioppo; Hailey Prohaska; Che Myong J. Ko

doi:10.1002/dvg.23084

Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse

Chan Jin Park, Guanglin Chen, Yongbum Koo, Po Ching P. Lin, Joseph A. Cacioppo, Hailey Prohaska, Che Myong J. Ko

Research output: Contribution to journal › Article › peer-review

Abstract

Two estrogen receptors, ESR1 and ESR2, are responsible for the classical actions of estrogens in mammalian species. They display different spatiotemporal expression patterns and nonoverlapping functions in various tissues and physiological conditions. In this study, a novel knock-in mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the natural Esr1 promoter (Esr1–iCre) was developed. Functional characterization of iCre expression by crossing them with reporter lines (ROSA26-lacZ or Ai9-RFP) showed that iCre is faithfully expressed in Esr1-lineage cells. This novel transgenic mouse line will be a useful animal model for lineage-tracing Esr1-expressing cells, selective gene ablation in the Esr1-lineage cells and for generating global Esr1 knockout mice.

Original language	English (US)
Article number	e23084
Journal	Genesis
Volume	55
Issue number	12
DOIs	https://doi.org/10.1002/dvg.23084
State	Published - Dec 2017

Keywords

BAC clone
estrogen receptor alpha
iCre recombinase
transgenic

ASJC Scopus subject areas

Genetics
Endocrinology
Cell Biology

Online availability

10.1002/dvg.23084

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title = "Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse",

abstract = "Two estrogen receptors, ESR1 and ESR2, are responsible for the classical actions of estrogens in mammalian species. They display different spatiotemporal expression patterns and nonoverlapping functions in various tissues and physiological conditions. In this study, a novel knock-in mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the natural Esr1 promoter (Esr1–iCre) was developed. Functional characterization of iCre expression by crossing them with reporter lines (ROSA26-lacZ or Ai9-RFP) showed that iCre is faithfully expressed in Esr1-lineage cells. This novel transgenic mouse line will be a useful animal model for lineage-tracing Esr1-expressing cells, selective gene ablation in the Esr1-lineage cells and for generating global Esr1 knockout mice.",

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AU - Chen, Guanglin

AU - Koo, Yongbum

AU - Lin, Po Ching P.

AU - Cacioppo, Joseph A.

AU - Prohaska, Hailey

AU - Ko, Che Myong J.

PY - 2017/12

Y1 - 2017/12

N2 - Two estrogen receptors, ESR1 and ESR2, are responsible for the classical actions of estrogens in mammalian species. They display different spatiotemporal expression patterns and nonoverlapping functions in various tissues and physiological conditions. In this study, a novel knock-in mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the natural Esr1 promoter (Esr1–iCre) was developed. Functional characterization of iCre expression by crossing them with reporter lines (ROSA26-lacZ or Ai9-RFP) showed that iCre is faithfully expressed in Esr1-lineage cells. This novel transgenic mouse line will be a useful animal model for lineage-tracing Esr1-expressing cells, selective gene ablation in the Esr1-lineage cells and for generating global Esr1 knockout mice.

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KW - BAC clone

KW - estrogen receptor alpha

KW - iCre recombinase

KW - transgenic

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Generation and characterization of an estrogen receptor alpha-iCre knock-in mouse

Abstract

Keywords

ASJC Scopus subject areas

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