Generation and characterization of an endothelin-2 iCre mouse

Joseph A. Cacioppo, Yongbum Koo, Po Ching Patrick Lin, Arnon Gal, Chemyong Ko

Research output: Contribution to journalArticle

Abstract

A novel transgenic mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the Endothelin-2 gene (edn2) promoter was developed for the conditional deletion of genes in Endothelin-2 lineage cells and for the spatial and temporal localization of Endothelin-2 expression. Endothelin-2 (EDN2, ET-2, previously VIC) is a transcriptionally regulated 21 amino acid peptide implicated in vascular homeostasis, and more recently in female reproduction, gastrointestinal function, immunology, and cancer pathogenesis that acts through membrane receptors and G-protein signaling. A cassette (edn2-iCre) was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker in front of the endogenous start codon of the edn2 gene in a mouse genome BAC clone. The cassette was introduced into the C57BL/6 genome by pronuclear injection, and two lines of edn2-iCre positive mice were produced. The edn2-iCre mice were bred with ROSA26-lacZ and Ai9 reporter mice to visualize areas of functional iCre expression. Strong expression was seen in the periovulatory ovary, stomach and small intestine, and colon. Uniquely, we report punctate expression in the corneal epithelium, the liver, the lung, the pituitary, the uterus, and the heart. In the embryo, expression is localized in developing hair follicles and the dermis. Therefore, edn2-iCre mice will serve as a novel line for conditional gene deletion in these tissues.

Original languageEnglish (US)
Pages (from-to)245-256
Number of pages12
JournalGenesis
Volume53
Issue number2
DOIs
StatePublished - Feb 1 2015

Fingerprint

Endothelin-2
Genes
Gene Deletion
Genome
Cre recombinase
Corneal Epithelium
Polyadenylation
Neomycin
Initiator Codon
Hair Follicle
Dermis
Allergy and Immunology
GTP-Binding Proteins
Codon
Transgenic Mice
Small Intestine
Uterus
Reproduction
Blood Vessels
Ovary

Keywords

  • BAC clone
  • Cre recombinase
  • Endothelin-2
  • Ovary
  • Transgenic

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Generation and characterization of an endothelin-2 iCre mouse. / Cacioppo, Joseph A.; Koo, Yongbum; Lin, Po Ching Patrick; Gal, Arnon; Ko, Chemyong.

In: Genesis, Vol. 53, No. 2, 01.02.2015, p. 245-256.

Research output: Contribution to journalArticle

Cacioppo, Joseph A. ; Koo, Yongbum ; Lin, Po Ching Patrick ; Gal, Arnon ; Ko, Chemyong. / Generation and characterization of an endothelin-2 iCre mouse. In: Genesis. 2015 ; Vol. 53, No. 2. pp. 245-256.
@article{a501886b330c4d54919b1df2e55e9e6c,
title = "Generation and characterization of an endothelin-2 iCre mouse",
abstract = "A novel transgenic mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the Endothelin-2 gene (edn2) promoter was developed for the conditional deletion of genes in Endothelin-2 lineage cells and for the spatial and temporal localization of Endothelin-2 expression. Endothelin-2 (EDN2, ET-2, previously VIC) is a transcriptionally regulated 21 amino acid peptide implicated in vascular homeostasis, and more recently in female reproduction, gastrointestinal function, immunology, and cancer pathogenesis that acts through membrane receptors and G-protein signaling. A cassette (edn2-iCre) was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker in front of the endogenous start codon of the edn2 gene in a mouse genome BAC clone. The cassette was introduced into the C57BL/6 genome by pronuclear injection, and two lines of edn2-iCre positive mice were produced. The edn2-iCre mice were bred with ROSA26-lacZ and Ai9 reporter mice to visualize areas of functional iCre expression. Strong expression was seen in the periovulatory ovary, stomach and small intestine, and colon. Uniquely, we report punctate expression in the corneal epithelium, the liver, the lung, the pituitary, the uterus, and the heart. In the embryo, expression is localized in developing hair follicles and the dermis. Therefore, edn2-iCre mice will serve as a novel line for conditional gene deletion in these tissues.",
keywords = "BAC clone, Cre recombinase, Endothelin-2, Ovary, Transgenic",
author = "Cacioppo, {Joseph A.} and Yongbum Koo and Lin, {Po Ching Patrick} and Arnon Gal and Chemyong Ko",
year = "2015",
month = "2",
day = "1",
doi = "10.1002/dvg.22845",
language = "English (US)",
volume = "53",
pages = "245--256",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Generation and characterization of an endothelin-2 iCre mouse

AU - Cacioppo, Joseph A.

AU - Koo, Yongbum

AU - Lin, Po Ching Patrick

AU - Gal, Arnon

AU - Ko, Chemyong

PY - 2015/2/1

Y1 - 2015/2/1

N2 - A novel transgenic mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the Endothelin-2 gene (edn2) promoter was developed for the conditional deletion of genes in Endothelin-2 lineage cells and for the spatial and temporal localization of Endothelin-2 expression. Endothelin-2 (EDN2, ET-2, previously VIC) is a transcriptionally regulated 21 amino acid peptide implicated in vascular homeostasis, and more recently in female reproduction, gastrointestinal function, immunology, and cancer pathogenesis that acts through membrane receptors and G-protein signaling. A cassette (edn2-iCre) was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker in front of the endogenous start codon of the edn2 gene in a mouse genome BAC clone. The cassette was introduced into the C57BL/6 genome by pronuclear injection, and two lines of edn2-iCre positive mice were produced. The edn2-iCre mice were bred with ROSA26-lacZ and Ai9 reporter mice to visualize areas of functional iCre expression. Strong expression was seen in the periovulatory ovary, stomach and small intestine, and colon. Uniquely, we report punctate expression in the corneal epithelium, the liver, the lung, the pituitary, the uterus, and the heart. In the embryo, expression is localized in developing hair follicles and the dermis. Therefore, edn2-iCre mice will serve as a novel line for conditional gene deletion in these tissues.

AB - A novel transgenic mouse line that expresses codon-improved Cre recombinase (iCre) under regulation of the Endothelin-2 gene (edn2) promoter was developed for the conditional deletion of genes in Endothelin-2 lineage cells and for the spatial and temporal localization of Endothelin-2 expression. Endothelin-2 (EDN2, ET-2, previously VIC) is a transcriptionally regulated 21 amino acid peptide implicated in vascular homeostasis, and more recently in female reproduction, gastrointestinal function, immunology, and cancer pathogenesis that acts through membrane receptors and G-protein signaling. A cassette (edn2-iCre) was constructed that contained iCre, a polyadenylation sequence, and a neomycin selection marker in front of the endogenous start codon of the edn2 gene in a mouse genome BAC clone. The cassette was introduced into the C57BL/6 genome by pronuclear injection, and two lines of edn2-iCre positive mice were produced. The edn2-iCre mice were bred with ROSA26-lacZ and Ai9 reporter mice to visualize areas of functional iCre expression. Strong expression was seen in the periovulatory ovary, stomach and small intestine, and colon. Uniquely, we report punctate expression in the corneal epithelium, the liver, the lung, the pituitary, the uterus, and the heart. In the embryo, expression is localized in developing hair follicles and the dermis. Therefore, edn2-iCre mice will serve as a novel line for conditional gene deletion in these tissues.

KW - BAC clone

KW - Cre recombinase

KW - Endothelin-2

KW - Ovary

KW - Transgenic

UR - http://www.scopus.com/inward/record.url?scp=84923197754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923197754&partnerID=8YFLogxK

U2 - 10.1002/dvg.22845

DO - 10.1002/dvg.22845

M3 - Article

C2 - 25604013

AN - SCOPUS:84923197754

VL - 53

SP - 245

EP - 256

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 2

ER -