General esterases of silkmoth moulting fluid: Preliminary characterization

Benita S. Katzenellenbogen, Fotis C. Kafatos

Research output: Contribution to journalArticle

Abstract

The moulting fluid of silkmoths contains not only proteolytic enzymes with amino acid esterase activity, but also two naphthyl esterases (general esterases). These enzymes have been partially purified and separated by sucrose density-gradient centrifugation and DEAE-cellulose column chromatography. One is weakly anionic at pH 8 and has a molecular weight of 64,000; the other is cationic and has a molecular weight of 57,000. Both enzymes are most stable under the mildly alkaline pH conditions characteristic of moulting fluid but are irreversibly inactivated at acid pH. Both are inhibited by diisopropyl fluorophosphate, although at different rates. The enzymes hydrolyse low molecular weight esters of aromatic alcohols, but lack proteolytic activity; inhibition and specificity studies suggest that they are carboxyl esterases. They are found throughout development, in both moulting gel and moulting fluid; their rôle in the moulting process is as yet unclear.

Original languageEnglish (US)
JournalJournal of insect physiology
Volume17
Issue number6
DOIs
StatePublished - Jun 1971
Externally publishedYes

Fingerprint

Molting
Bombyx
Esterases
esterases
molting
Molecular Weight
fluorophosphate
molecular weight
enzymes
Enzymes
Acetylesterase
DEAE-Cellulose Chromatography
Density Gradient Centrifugation
aromatic compounds
Sucrose
chromatography
Esters
cellulose
Peptide Hydrolases
alcohols

ASJC Scopus subject areas

  • Physiology
  • Insect Science

Cite this

General esterases of silkmoth moulting fluid : Preliminary characterization. / Katzenellenbogen, Benita S.; Kafatos, Fotis C.

In: Journal of insect physiology, Vol. 17, No. 6, 06.1971.

Research output: Contribution to journalArticle

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