General deoxyribozyme-catalyzed synthesis of native 3′-5′ RNA linkages

Whitney E. Purtha, Rebecca L. Coppins, Mary K. Smalley, Scott K Silverman

Research output: Contribution to journalArticle

Abstract

An elusive goal for nucleic acid enzymology has been deoxyribozymes that ligate RNA rapidly, sequence-generally, with formation of native 3′-5′ linkages, and in preparatively useful yield. Using in vitro selection, we have identified Mg2+- and Zn2+-dependent deoxyribozymes that simultaneously fulfill all four of these criteria. The new deoxyribozymes operate under practical incubation conditions and have modest RNA substrate sequence requirements, specifically D↓RA for 9DB1 and A↓R for 7DE5 (D = A, G, or U; R = A or G). These requirements are comparable to those of deoxyribozymes such as 10-23 and 8-17, which are already widely used as biochemical tools for RNA cleavage. We anticipate that the 9DB1 and 7DE5 deoxyribozymes will find immediate practical application for RNA ligation.

Original languageEnglish (US)
Pages (from-to)13124-13125
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number38
DOIs
StatePublished - Sep 28 2005

Fingerprint

Catalytic DNA
RNA
RNA Cleavage
nucleic acid
cleavage
Nucleic acids
incubation
Nucleic Acids
Ligation
substrate
Substrates

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

General deoxyribozyme-catalyzed synthesis of native 3′-5′ RNA linkages. / Purtha, Whitney E.; Coppins, Rebecca L.; Smalley, Mary K.; Silverman, Scott K.

In: Journal of the American Chemical Society, Vol. 127, No. 38, 28.09.2005, p. 13124-13125.

Research output: Contribution to journalArticle

Purtha, Whitney E. ; Coppins, Rebecca L. ; Smalley, Mary K. ; Silverman, Scott K. / General deoxyribozyme-catalyzed synthesis of native 3′-5′ RNA linkages. In: Journal of the American Chemical Society. 2005 ; Vol. 127, No. 38. pp. 13124-13125.
@article{aacf3c5edf3b4b038e4b7ee1cc346cb7,
title = "General deoxyribozyme-catalyzed synthesis of native 3′-5′ RNA linkages",
abstract = "An elusive goal for nucleic acid enzymology has been deoxyribozymes that ligate RNA rapidly, sequence-generally, with formation of native 3′-5′ linkages, and in preparatively useful yield. Using in vitro selection, we have identified Mg2+- and Zn2+-dependent deoxyribozymes that simultaneously fulfill all four of these criteria. The new deoxyribozymes operate under practical incubation conditions and have modest RNA substrate sequence requirements, specifically D↓RA for 9DB1 and A↓R for 7DE5 (D = A, G, or U; R = A or G). These requirements are comparable to those of deoxyribozymes such as 10-23 and 8-17, which are already widely used as biochemical tools for RNA cleavage. We anticipate that the 9DB1 and 7DE5 deoxyribozymes will find immediate practical application for RNA ligation.",
author = "Purtha, {Whitney E.} and Coppins, {Rebecca L.} and Smalley, {Mary K.} and Silverman, {Scott K}",
year = "2005",
month = "9",
day = "28",
doi = "10.1021/ja0533702",
language = "English (US)",
volume = "127",
pages = "13124--13125",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "38",

}

TY - JOUR

T1 - General deoxyribozyme-catalyzed synthesis of native 3′-5′ RNA linkages

AU - Purtha, Whitney E.

AU - Coppins, Rebecca L.

AU - Smalley, Mary K.

AU - Silverman, Scott K

PY - 2005/9/28

Y1 - 2005/9/28

N2 - An elusive goal for nucleic acid enzymology has been deoxyribozymes that ligate RNA rapidly, sequence-generally, with formation of native 3′-5′ linkages, and in preparatively useful yield. Using in vitro selection, we have identified Mg2+- and Zn2+-dependent deoxyribozymes that simultaneously fulfill all four of these criteria. The new deoxyribozymes operate under practical incubation conditions and have modest RNA substrate sequence requirements, specifically D↓RA for 9DB1 and A↓R for 7DE5 (D = A, G, or U; R = A or G). These requirements are comparable to those of deoxyribozymes such as 10-23 and 8-17, which are already widely used as biochemical tools for RNA cleavage. We anticipate that the 9DB1 and 7DE5 deoxyribozymes will find immediate practical application for RNA ligation.

AB - An elusive goal for nucleic acid enzymology has been deoxyribozymes that ligate RNA rapidly, sequence-generally, with formation of native 3′-5′ linkages, and in preparatively useful yield. Using in vitro selection, we have identified Mg2+- and Zn2+-dependent deoxyribozymes that simultaneously fulfill all four of these criteria. The new deoxyribozymes operate under practical incubation conditions and have modest RNA substrate sequence requirements, specifically D↓RA for 9DB1 and A↓R for 7DE5 (D = A, G, or U; R = A or G). These requirements are comparable to those of deoxyribozymes such as 10-23 and 8-17, which are already widely used as biochemical tools for RNA cleavage. We anticipate that the 9DB1 and 7DE5 deoxyribozymes will find immediate practical application for RNA ligation.

UR - http://www.scopus.com/inward/record.url?scp=25444501848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25444501848&partnerID=8YFLogxK

U2 - 10.1021/ja0533702

DO - 10.1021/ja0533702

M3 - Article

C2 - 16173722

AN - SCOPUS:25444501848

VL - 127

SP - 13124

EP - 13125

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 38

ER -