Gene-specific characterization of human histone H2B by electron capture dissociation

Nertila Siuti, Michael J. Roth, Craig A. Mizzen, Neil L. Kelleher, James J. Pesavento

Research output: Contribution to journalArticle

Abstract

The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalJournal of Proteome Research
Volume5
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

Histones
Genes
Cells
Electrons
Butyric Acid
Colchicine
G1 Phase
HeLa Cells
Mitosis
Mass spectrometry
Mass Spectrometry
Cell Cycle
Protein Isoforms
Proteins

Keywords

  • Chromatin
  • Electron capture dissociation
  • Histone
  • Post-translational modifications
  • Top down mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Siuti, N., Roth, M. J., Mizzen, C. A., Kelleher, N. L., & Pesavento, J. J. (2006). Gene-specific characterization of human histone H2B by electron capture dissociation. Journal of Proteome Research, 5(2), 233-239. https://doi.org/10.1021/pr050268v

Gene-specific characterization of human histone H2B by electron capture dissociation. / Siuti, Nertila; Roth, Michael J.; Mizzen, Craig A.; Kelleher, Neil L.; Pesavento, James J.

In: Journal of Proteome Research, Vol. 5, No. 2, 01.02.2006, p. 233-239.

Research output: Contribution to journalArticle

Siuti, N, Roth, MJ, Mizzen, CA, Kelleher, NL & Pesavento, JJ 2006, 'Gene-specific characterization of human histone H2B by electron capture dissociation', Journal of Proteome Research, vol. 5, no. 2, pp. 233-239. https://doi.org/10.1021/pr050268v
Siuti, Nertila ; Roth, Michael J. ; Mizzen, Craig A. ; Kelleher, Neil L. ; Pesavento, James J. / Gene-specific characterization of human histone H2B by electron capture dissociation. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 2. pp. 233-239.
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