Gene regulation by low level UV-B radiation: Identification by DNA array analysis

Mikael Brosché, Mary A Schuler, Irina Kalbina, Lynn Connor, Åke Strid

Research output: Contribution to journalArticle

Abstract

UV-B radiation alters transcript levels of various defence genes and photosynthetic genes in plants. Utilising a DNA array with 5000 ESTs and cDNAs from Arabidopsis thaliana, 70 genes were found to show a greater than two-fold induction or repression of transcript levels. Six genes (MEB5.2, PyroA, Ubq3, Lhcb6, F5D21.10 and the gene for an RNA polymerase II subunit) were tested for stress specific gene regulation on northern blots with RNA from plants exposed to low dose UV-B radiation, ozone or wounding. Transcript levels for PyroA, Ubq3 and the gene for a RNA polymerase II subunit were all specifically increased by UV-B. MEB5.2 mRNA levels also rose, whereas Lhcb6 and F5D21.10 transcript levels decreased under all stresses. The PyroA gene product in fungi is needed for biosynthesis of pyridoxine, and might have a role in protection against singlet oxygen. The Ubq3 gene encodes the ubiquitin protein that is attached to proteins destined for degradation. MEB5.2 and F5D21.10 represent novel gene products whose function have not yet been identified. Pairwise comparisons between the UV-B inducible promoters have identified a series of elements present in the MEB5.2 and PyroA promoters, absent from promoters of genes for early phenylpropanoid metabolism and that may be responsible for modulating their UV-B responses.

Original languageEnglish (US)
Pages (from-to)656-664
Number of pages9
JournalPhotochemical and Photobiological Sciences
Volume1
Issue number9
DOIs
StatePublished - Sep 1 2002

Fingerprint

gene expression
Oligonucleotide Array Sequence Analysis
Gene expression
genes
deoxyribonucleic acid
Genes
Radiation
DNA
radiation
RNA Polymerase II
pyridoxine
Plant RNA
proteins
Plant Genes
Pyridoxine
Singlet Oxygen
biosynthesis
fungi
Ozone
Biosynthesis

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Gene regulation by low level UV-B radiation : Identification by DNA array analysis. / Brosché, Mikael; Schuler, Mary A; Kalbina, Irina; Connor, Lynn; Strid, Åke.

In: Photochemical and Photobiological Sciences, Vol. 1, No. 9, 01.09.2002, p. 656-664.

Research output: Contribution to journalArticle

Brosché, Mikael ; Schuler, Mary A ; Kalbina, Irina ; Connor, Lynn ; Strid, Åke. / Gene regulation by low level UV-B radiation : Identification by DNA array analysis. In: Photochemical and Photobiological Sciences. 2002 ; Vol. 1, No. 9. pp. 656-664.
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