Gene expression profiling: Opening the black box of plant ecosystem responses to global change

Andrew Leakey, Elizabeth Ainsworth, Stephanie M. Bernard, R. J.Cody Markelz, Donald Richard Ort, Sarah A. Placella, Alistair Rogers, Melinda D. Smith, Erika A. Sudderth, David J. Weston, Stan D. Wullschleger, Shenghua Yuan

Research output: Contribution to journalArticle

Abstract

The use of genomic techniques to address ecological questions is emerging as the field of genomic ecology. Experimentation under environmentally realistic conditions to investigate the molecular response of plants to meaningful changes in growth conditions and ecological interactions is the defining feature of genomic ecology. Because the impact of global change factors on plant performance are mediated by direct effects at the molecular, biochemical, and physiological scales, gene expression analysis promises important advances in understanding factors that have previously been consigned to the 'black box' of unknown mechanism. Various tools and approaches are available for assessing gene expression in model and nonmodel species as part of global change biology studies. Each approach has its own unique advantages and constraints. A first generation of genomic ecology studies in managed ecosystems and mesocosms have provided a testbed for the approach and have begun to reveal how the experimental design and data analysis of gene expression studies can be tailored for use in an ecological context.

Original languageEnglish (US)
Pages (from-to)1201-1213
Number of pages13
JournalGlobal change biology
Volume15
Issue number5
DOIs
StatePublished - Apr 23 2009

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ecosystem response
Ecology
Gene expression
global change
Ecosystems
gene expression
genomics
ecology
Testbeds
Design of experiments
experimental design
ecosystem

Keywords

  • Elevated CO
  • Genomic
  • Microarray

ASJC Scopus subject areas

  • Global and Planetary Change
  • Environmental Chemistry
  • Ecology
  • Environmental Science(all)

Cite this

Gene expression profiling : Opening the black box of plant ecosystem responses to global change. / Leakey, Andrew; Ainsworth, Elizabeth; Bernard, Stephanie M.; Markelz, R. J.Cody; Ort, Donald Richard; Placella, Sarah A.; Rogers, Alistair; Smith, Melinda D.; Sudderth, Erika A.; Weston, David J.; Wullschleger, Stan D.; Yuan, Shenghua.

In: Global change biology, Vol. 15, No. 5, 23.04.2009, p. 1201-1213.

Research output: Contribution to journalArticle

Leakey, A, Ainsworth, E, Bernard, SM, Markelz, RJC, Ort, DR, Placella, SA, Rogers, A, Smith, MD, Sudderth, EA, Weston, DJ, Wullschleger, SD & Yuan, S 2009, 'Gene expression profiling: Opening the black box of plant ecosystem responses to global change', Global change biology, vol. 15, no. 5, pp. 1201-1213. https://doi.org/10.1111/j.1365-2486.2008.01818.x
Leakey, Andrew ; Ainsworth, Elizabeth ; Bernard, Stephanie M. ; Markelz, R. J.Cody ; Ort, Donald Richard ; Placella, Sarah A. ; Rogers, Alistair ; Smith, Melinda D. ; Sudderth, Erika A. ; Weston, David J. ; Wullschleger, Stan D. ; Yuan, Shenghua. / Gene expression profiling : Opening the black box of plant ecosystem responses to global change. In: Global change biology. 2009 ; Vol. 15, No. 5. pp. 1201-1213.
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