Gene expression profiles of lens regeneration and development in Xenopus laevis

Erica L. Malloch, Kimberly J. Perry, Lisa Fukui, Verity R. Johnson, Jason Wever, Caroline W. Beck, Michael W. King, Jonathan Joseph Henry

Research output: Contribution to journalArticle

Abstract

Seven hundred and thirty-four unique genes were recovered from a cDNA library enriched for genes up-regulated during the process of lens regeneration in the frog Xenopus laevis. The sequences represent transcription factors, proteins involved in RNA synthesis/processing, components of prominent cell signaling pathways, genes involved in protein processing, transport, and degradation (e.g., the ubiquitin/proteasome pathway), matrix metalloproteases (MMPs), as well as many other proteins. The findings implicate specific signal transduction pathways in the process of lens regeneration, including the FGF, TGF-beta, MAPK, Retinoic acid, Wnt, and hedgehog signaling pathways, which are known to play important roles in eye/lens development and regeneration in various systems. In situ hybridization revealed that the majority of genes recovered are expressed during embryogenesis, including in eye tissues. Several novel genes specifically expressed in lenses were identified. The suite of genes was compared to those up-regulated in other regenerating tissues/organisms, and a small degree of overlap was detected.

Original languageEnglish (US)
Pages (from-to)2340-2356
Number of pages17
JournalDevelopmental Dynamics
Volume238
Issue number9
DOIs
StatePublished - Sep 21 2009

Fingerprint

Xenopus laevis
Transcriptome
Lenses
Regeneration
Genes
Crystalline Lens
Wnt Signaling Pathway
Hedgehogs
Metalloproteases
Protein Transport
Proteasome Endopeptidase Complex
Cellular Structures
Ubiquitin
Tretinoin
Gene Library
Transforming Growth Factor beta
Anura
Proteolysis
Embryonic Development
In Situ Hybridization

Keywords

  • Development
  • Eye
  • Lens
  • Regeneration
  • Transdifferentiation
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Malloch, E. L., Perry, K. J., Fukui, L., Johnson, V. R., Wever, J., Beck, C. W., ... Henry, J. J. (2009). Gene expression profiles of lens regeneration and development in Xenopus laevis. Developmental Dynamics, 238(9), 2340-2356. https://doi.org/10.1002/dvdy.21998

Gene expression profiles of lens regeneration and development in Xenopus laevis. / Malloch, Erica L.; Perry, Kimberly J.; Fukui, Lisa; Johnson, Verity R.; Wever, Jason; Beck, Caroline W.; King, Michael W.; Henry, Jonathan Joseph.

In: Developmental Dynamics, Vol. 238, No. 9, 21.09.2009, p. 2340-2356.

Research output: Contribution to journalArticle

Malloch, EL, Perry, KJ, Fukui, L, Johnson, VR, Wever, J, Beck, CW, King, MW & Henry, JJ 2009, 'Gene expression profiles of lens regeneration and development in Xenopus laevis', Developmental Dynamics, vol. 238, no. 9, pp. 2340-2356. https://doi.org/10.1002/dvdy.21998
Malloch EL, Perry KJ, Fukui L, Johnson VR, Wever J, Beck CW et al. Gene expression profiles of lens regeneration and development in Xenopus laevis. Developmental Dynamics. 2009 Sep 21;238(9):2340-2356. https://doi.org/10.1002/dvdy.21998
Malloch, Erica L. ; Perry, Kimberly J. ; Fukui, Lisa ; Johnson, Verity R. ; Wever, Jason ; Beck, Caroline W. ; King, Michael W. ; Henry, Jonathan Joseph. / Gene expression profiles of lens regeneration and development in Xenopus laevis. In: Developmental Dynamics. 2009 ; Vol. 238, No. 9. pp. 2340-2356.
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