Abstract

A human cell line was gene-edited to provide a visually observable fluorescence response to extracellular free calcium using the CRISPR-Cas9 system. The developed sensor cells have a dynamic range of 0.01-10 μM, and the ability to be reused for multiple signal excitations. We envision using gene-edited cells as responsive elements within tissue engineered constructs that interface with the body for noninvasive semi-continuous monitoring of circulating analytes.

Original languageEnglish (US)
Title of host publication2019 IEEE Sensors, SENSORS 2019 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728116341
DOIs
StatePublished - Oct 2019
Event18th IEEE Sensors, SENSORS 2019 - Montreal, Canada
Duration: Oct 27 2019Oct 30 2019

Publication series

NameProceedings of IEEE Sensors
Volume2019-October
ISSN (Print)1930-0395
ISSN (Electronic)2168-9229

Conference

Conference18th IEEE Sensors, SENSORS 2019
CountryCanada
CityMontreal
Period10/27/1910/30/19

Keywords

  • calcium
  • CRISPR-Cas9
  • live cell sensor

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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  • Cite this

    Sun, F., Lee, D. H., Gaj, T., & Cunningham, B. T. (2019). Gene-Edited Live Cell Sensor for Free Calcium. In 2019 IEEE Sensors, SENSORS 2019 - Conference Proceedings [8956634] (Proceedings of IEEE Sensors; Vol. 2019-October). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SENSORS43011.2019.8956634