Gene delivery method using photo-responsive poly(β-amino ester) as vectors

Nan Zheng; Yang Liu; Jianjun Cheng

doi:10.1007/978-1-4939-3718-9_16

Gene delivery method using photo-responsive poly(β-amino ester) as vectors

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral gene delivery vectors, forming the polymer/NA complexes for the intracellular transportation and release of the genetic materials into the target mammalian cells. Here we describe the poly(β-amino ester) (PBAE) with the photo-responsive domain built in the polymers, as a UV-light-responsive nonviral gene delivery vector to deliver and release plasmid DNA (pDNA) into HeLa cells and achieve enhanced transfection efficiency.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	259-267
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-4939-3718-9_16
State	Published - Jan 1 2016

Publication series

Name	Methods in Molecular Biology
Volume	1445
ISSN (Print)	1064-3745

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Keywords

Nonviral gene delivery
Photo-responsive
Poly (β-amino ester)s
Transfection efficiency

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Zheng, N., Liu, Y., & Cheng, J. (2016). Gene delivery method using photo-responsive poly(β-amino ester) as vectors. In Methods in Molecular Biology (pp. 259-267). (Methods in Molecular Biology; Vol. 1445). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3718-9_16

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Access to Document

10.1007/978-1-4939-3718-9_16