Abstract
Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral gene delivery vectors, forming the polymer/NA complexes for the intracellular transportation and release of the genetic materials into the target mammalian cells. Here we describe the poly(β-amino ester) (PBAE) with the photo-responsive domain built in the polymers, as a UV-light-responsive nonviral gene delivery vector to deliver and release plasmid DNA (pDNA) into HeLa cells and achieve enhanced transfection efficiency.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 259-267 |
| Number of pages | 9 |
| DOIs | |
| State | Published - Jan 1 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1445 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Nonviral gene delivery
- Photo-responsive
- Poly (β-amino ester)s
- Transfection efficiency
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Gene delivery method using photo-responsive poly(β-amino ester) as vectors. / Zheng, Nan; Liu, Yang; Cheng, Jianjun.
Methods in Molecular Biology. Humana Press Inc., 2016. p. 259-267 (Methods in Molecular Biology; Vol. 1445).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Gene delivery method using photo-responsive poly(β-amino ester) as vectors
AU - Zheng, Nan
AU - Liu, Yang
AU - Cheng, Jianjun
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral gene delivery vectors, forming the polymer/NA complexes for the intracellular transportation and release of the genetic materials into the target mammalian cells. Here we describe the poly(β-amino ester) (PBAE) with the photo-responsive domain built in the polymers, as a UV-light-responsive nonviral gene delivery vector to deliver and release plasmid DNA (pDNA) into HeLa cells and achieve enhanced transfection efficiency.
AB - Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral gene delivery vectors, forming the polymer/NA complexes for the intracellular transportation and release of the genetic materials into the target mammalian cells. Here we describe the poly(β-amino ester) (PBAE) with the photo-responsive domain built in the polymers, as a UV-light-responsive nonviral gene delivery vector to deliver and release plasmid DNA (pDNA) into HeLa cells and achieve enhanced transfection efficiency.
KW - Nonviral gene delivery
KW - Photo-responsive
KW - Poly (β-amino ester)s
KW - Transfection efficiency
UR - http://www.scopus.com/inward/record.url?scp=84979266081&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84979266081&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3718-9_16
DO - 10.1007/978-1-4939-3718-9_16
M3 - Chapter
C2 - 27436324
AN - SCOPUS:84979266081
T3 - Methods in Molecular Biology
SP - 259
EP - 267
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -