Abstract

Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral gene delivery vectors, forming the polymer/NA complexes for the intracellular transportation and release of the genetic materials into the target mammalian cells. Here we describe the poly(β-amino ester) (PBAE) with the photo-responsive domain built in the polymers, as a UV-light-responsive nonviral gene delivery vector to deliver and release plasmid DNA (pDNA) into HeLa cells and achieve enhanced transfection efficiency.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages259-267
Number of pages9
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1445
ISSN (Print)1064-3745

Fingerprint

Esters
Polymers
Nucleic Acids
Genes
Transfection
Ultraviolet Rays
HeLa Cells
Plasmids
DNA

Keywords

  • Nonviral gene delivery
  • Photo-responsive
  • Poly (β-amino ester)s
  • Transfection efficiency

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Zheng, N., Liu, Y., & Cheng, J. (2016). Gene delivery method using photo-responsive poly(β-amino ester) as vectors. In Methods in Molecular Biology (pp. 259-267). (Methods in Molecular Biology; Vol. 1445). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3718-9_16

Gene delivery method using photo-responsive poly(β-amino ester) as vectors. / Zheng, Nan; Liu, Yang; Cheng, Jianjun.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 259-267 (Methods in Molecular Biology; Vol. 1445).

Research output: Chapter in Book/Report/Conference proceedingChapter

Zheng, N, Liu, Y & Cheng, J 2016, Gene delivery method using photo-responsive poly(β-amino ester) as vectors. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1445, Humana Press Inc., pp. 259-267. https://doi.org/10.1007/978-1-4939-3718-9_16
Zheng N, Liu Y, Cheng J. Gene delivery method using photo-responsive poly(β-amino ester) as vectors. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 259-267. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3718-9_16
Zheng, Nan ; Liu, Yang ; Cheng, Jianjun. / Gene delivery method using photo-responsive poly(β-amino ester) as vectors. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 259-267 (Methods in Molecular Biology).
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