Abstract
Nonviral vectors show great potential in delivering nucleic acids (NA) into many mammalian cells to achieve efficient gene transfection. Among these, cationic polymer is one of the most widely used nonviral gene delivery vectors, forming the polymer/NA complexes for the intracellular transportation and release of the genetic materials into the target mammalian cells. Here we describe the poly(β-amino ester) (PBAE) with the photo-responsive domain built in the polymers, as a UV-light-responsive nonviral gene delivery vector to deliver and release plasmid DNA (pDNA) into HeLa cells and achieve enhanced transfection efficiency.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 259-267 |
| Number of pages | 9 |
| DOIs | |
| State | Published - Jan 1 2016 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1445 |
| ISSN (Print) | 1064-3745 |
Keywords
- Nonviral gene delivery
- Photo-responsive
- Poly (β-amino ester)s
- Transfection efficiency
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Zheng, N., Liu, Y., & Cheng, J. (2016). Gene delivery method using photo-responsive poly(β-amino ester) as vectors. In Methods in Molecular Biology (pp. 259-267). (Methods in Molecular Biology; Vol. 1445). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3718-9_16