Gender-related expression of TRα and TRβ in the protandrous black porgy, Acanthopagrus schlegeli, during sex change processes

Kwang Wook An, Myung In An, Erik R. Nelson, Hamid R. Habibi, Cheol Young Choi

Research output: Contribution to journalArticle

Abstract

We cloned the thyroid hormone receptor α (TRα) and β (TRβ) cDNAs from the ovaries of the protandrous black porgy and compared the expression levels of TRα and TRβ mRNA during the sex change in black porgy. We observed that the TRα mRNA by quantitative real-time PCR and protein levels by Western blot were highest in the mature ovaries. Additionally, TRβ mRNA levels were only expressed highly in the mature ovaries when compared to any other gonadal stages. Then, we injected gonadotropin-releasing hormone analogue (GnRHa) to know the effects on TRs mRNA in immature black porgy. Injection with GnRHa resulted in a significant increase in TRα level while significantly reducing TRβ level after 12 h. We concluded that TRα was related in testicular development as well as ovarian development and TRβ was only affect to ovarian development in black porgy. These results will provide a framework for better understanding of the role of TRs during sex change processes in this fish.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume165
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

porgy
Acanthopagrus schlegelii schlegelii
Thyroid Hormone Receptors
sex reversal
gender
synthetic hormones
Ovary
Messenger RNA
ovarian development
gonadotropin-releasing hormone
Gonadotropin-Releasing Hormone
thyroid hormone receptors
testicular development
Real-Time Polymerase Chain Reaction
Fishes
quantitative polymerase chain reaction
Western blotting
Complementary DNA

Keywords

  • Black porgy
  • Sex change
  • Thyroid hormone receptor α
  • Thyroid hormone receptor β

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

Cite this

Gender-related expression of TRα and TRβ in the protandrous black porgy, Acanthopagrus schlegeli, during sex change processes. / An, Kwang Wook; An, Myung In; Nelson, Erik R.; Habibi, Hamid R.; Choi, Cheol Young.

In: General and Comparative Endocrinology, Vol. 165, No. 1, 01.01.2010, p. 11-18.

Research output: Contribution to journalArticle

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