@article{6a48623307b645b38b50fe61ba7245aa,
title = "Gelatin maleimide microgels for hematopoietic progenitor cell encapsulation",
abstract = "Hematopoietic stem cells (HSCs) are the apical cells of the hematopoietic system, giving rise to cells of the blood and lymph lineages. HSCs reside primarily within bone marrow niches that contain matrix and cell-derived signals that help inform stem cell fate. Aspects of the bone marrow microenvironment have been captured in vitro by encapsulating cells within hydrogel matrices that mimic native mechanical and biochemical properties. Hydrogel microparticles, or microgels, are increasingly being used to assemble granular biomaterials for cell culture and noninvasive delivery applications. Here, we report the optimization of a gelatin maleimide hydrogel system to create monodisperse gelatin microgels via a flow-focusing microfluidic process. We report characteristic hydrogel stiffness, stability, and swelling characteristics as well as encapsulation of murine hematopoietic stem and progenitor cells, and mesenchymal stem cells within microgels. Microgels support cell viability, confirming compatibility of the microfluidic encapsulation process with these sensitive bone marrow cell populations. Overall, this work presents a microgel-based gelatin maleimide hydrogel as a foundation for future development of a multicellular artificial bone marrow culture system.",
keywords = "bone marrow, gelatin, hematopoietic stem cell, microgel",
author = "Thompson, {Gunnar B.} and Gilchrist, {Aidan E.} and Lam, {Vincent M.} and Nunes, {Alison C.} and Payan, {Brittany A.} and Ana Mora-Boza and Serrano, {Julio F.} and Garc{\'i}a, {Andr{\'e}s J.} and Harley, {Brendan A.C.}",
note = "Funding sources include the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Number 2 R01 DK099528 (BACH), R56 DK099528,\u00A0R21 DK131751 (BACH), and F31 DK117514 (AEG); the National Cancer Institutes of the National Institutes of Health under Award Number R01 CA256481 (BACH); and the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under Award Number T32 EB019944. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. The authors are also grateful for additional funding provided by the Department of Chemical & Biomolecular Engineering, the Carl R. Woese Institute for Genomic Biology, the Cancer Center at Illinois, and the Illinois Scholars Undergraduate Research Program at the University of Illinois Urbana-Champaign. A.M.B. acknowledges support from EN Horizon Europe Work Programme 2021-2022 under grant agreement Number 101028216 - Synmat For Organoids. Funding sources include the National Institute of Diabetes and Digestive and Kidney Diseases of the National Institutes of Health under Award Number 2 R01 DK099528 (BACH), R56 DK099528, R21 DK131751 (BACH), and F31 DK117514 (AEG); the National Cancer Institutes of the National Institutes of Health under Award Number R01 CA256481 (BACH); and the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under Award Number T32 EB019944. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. The authors are also grateful for additional funding provided by the Department of Chemical & Biomolecular Engineering, the Carl R. Woese Institute for Genomic Biology, the Cancer Center at Illinois, and the Illinois Scholars Undergraduate Research Program at the University of Illinois Urbana\u2010Champaign. A.M.B. acknowledges support from EN Horizon Europe Work Programme 2021\u20102022 under grant agreement Number 101028216 \u2010 Synmat For Organoids.",
year = "2024",
month = dec,
doi = "10.1002/jbm.a.37765",
language = "English (US)",
volume = "112",
pages = "2124--2135",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley & Sons, Ltd.",
number = "12",
}