Abstract
Statement of Purpose: A current and critical challenge in medicine and biology is understanding and replicating the array of cues that are responsible for controlling stem cell behavior. A major bottleneck is that it remains unclear how synergies and hierarchies between multiple niche signals affect the heterogeneity of stem cell response. Synthetic niches consisting of three-dimensional (3D) biomaterials have the potential to provide structural and molecular cues to guide and trigger a desired stem cell behavior. Thus, it is paramount to develop functional synthetic niches and technologies that allow the temporal tracking of niche remodeling and stem cell fate heterogeneity. Here, we report a gelatin-based hydrogel, adapting maleimide-thiol crosslinking chemistries from a PEG-based system1 to generate libraries of miniaturized synthetic niches surrounding stem cells with defined cellular, structural, and biomolecular signals via a droplet microfluidic technique. This will enable the incorporation of instructive biomaterial networks within the individual droplets to make tractable a wide range of questions regarding how niche signals shape hematopoietic cell identity. Extensions of this effort will bring new precision to the design of synthetic stem cell niches to address a wider variety of traumatic injuries and degenerative diseases.
| Original language | English (US) |
|---|---|
| Title of host publication | Society for Biomaterials Annual Meeting and Exposition 2019 |
| Subtitle of host publication | The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting |
| Publisher | Society for Biomaterials |
| Number of pages | 1 |
| ISBN (Electronic) | 9781510883901 |
| State | Published - Jan 1 2019 |
| Event | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States Duration: Apr 3 2019 → Apr 6 2019 |
Publication series
| Name | Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium |
|---|---|
| Volume | 40 |
| ISSN (Print) | 1526-7547 |
Conference
| Conference | 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence |
|---|---|
| Country | United States |
| City | Seattle |
| Period | 4/3/19 → 4/6/19 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Biotechnology
- Biomaterials
- Materials Chemistry
Cite this
Gelatin-based thiol/disulfide degradable hydrogels to encapsulate cells. / Serrano, Julio F.; Gilchrist, Aidan E.; García, Andrés J.; Harley, Brendan A.
Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Gelatin-based thiol/disulfide degradable hydrogels to encapsulate cells
AU - Serrano, Julio F.
AU - Gilchrist, Aidan E.
AU - García, Andrés J.
AU - Harley, Brendan A
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Statement of Purpose: A current and critical challenge in medicine and biology is understanding and replicating the array of cues that are responsible for controlling stem cell behavior. A major bottleneck is that it remains unclear how synergies and hierarchies between multiple niche signals affect the heterogeneity of stem cell response. Synthetic niches consisting of three-dimensional (3D) biomaterials have the potential to provide structural and molecular cues to guide and trigger a desired stem cell behavior. Thus, it is paramount to develop functional synthetic niches and technologies that allow the temporal tracking of niche remodeling and stem cell fate heterogeneity. Here, we report a gelatin-based hydrogel, adapting maleimide-thiol crosslinking chemistries from a PEG-based system1 to generate libraries of miniaturized synthetic niches surrounding stem cells with defined cellular, structural, and biomolecular signals via a droplet microfluidic technique. This will enable the incorporation of instructive biomaterial networks within the individual droplets to make tractable a wide range of questions regarding how niche signals shape hematopoietic cell identity. Extensions of this effort will bring new precision to the design of synthetic stem cell niches to address a wider variety of traumatic injuries and degenerative diseases.
AB - Statement of Purpose: A current and critical challenge in medicine and biology is understanding and replicating the array of cues that are responsible for controlling stem cell behavior. A major bottleneck is that it remains unclear how synergies and hierarchies between multiple niche signals affect the heterogeneity of stem cell response. Synthetic niches consisting of three-dimensional (3D) biomaterials have the potential to provide structural and molecular cues to guide and trigger a desired stem cell behavior. Thus, it is paramount to develop functional synthetic niches and technologies that allow the temporal tracking of niche remodeling and stem cell fate heterogeneity. Here, we report a gelatin-based hydrogel, adapting maleimide-thiol crosslinking chemistries from a PEG-based system1 to generate libraries of miniaturized synthetic niches surrounding stem cells with defined cellular, structural, and biomolecular signals via a droplet microfluidic technique. This will enable the incorporation of instructive biomaterial networks within the individual droplets to make tractable a wide range of questions regarding how niche signals shape hematopoietic cell identity. Extensions of this effort will bring new precision to the design of synthetic stem cell niches to address a wider variety of traumatic injuries and degenerative diseases.
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M3 - Conference contribution
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
ER -