TY - JOUR
T1 - Gas chromatography-mass spectrometry of biosynthetic 1H- 2H hybrid fatty acid methyl esters
AU - Oldfield, E.
N1 - Funding Information:
In addition, ions of the general formula [(CH,),CO,Me]+ with n = 2, 6, 10, were apparent in both spectra e.g. for n = 6, m/e = 151 (1) corresponds to [(C1H,),(C2H,),C0,Me]+ and m/e = 147 (2) corresponds to [(C1H,),(C2H,),C0,Me]+. These findings confirm that chain elongation has occurred from the a-carbon, and are in accord with the findings of Pollack and Toutellotte7 that A. Eaidlawii B supplemented with shorter chain acids (e.g. n-C,, C,, and Clo) increase production of palmitic acid, by chain elongation. Utilisation of deuterium labelled precursors together with capillary g.c.-m.s. to resolve and analyse quantitatively isotope hybrid molecules (using e.g. AVA techniques) may prove a useful tool on further related biosynthetic studies. I thank Professor J. A. McCloskey for the DCALC program, Dr. D. G. ff. Edward for the strain of A. laidlawii B (PG9), and the S.R.C. for financial support. (Received, 17th April 1972; Corn. 637.) N. Dinh-Nguyh and E. A. Stenhagen, Fr. P. 1,466,088; Chem. Abs., 1967, 67, 63,814. G. Wendt and J. A. McCloskey, Biochemistry, 1970, 9, 4854. S. Razin, H. J. Morowitz, and T. M. Terry, Proc. Nut. Acad. Sci. U.S.A., 1965, 54, 219. C. V. Hendrikson and C. Panos, Biochemistvy, 1969, 8, 646. J. A. McCloskey, A. M. Lawson, and F. A. J. M. Leemans, Chem. Comm., 1967, 285. F. W. McLafferty,AnaZyt. Chem., 1956, 28, 306; N. Dinh-Nguyh, Arkiv. Kemi, 1968, 28, 289. J. D. Pollack and M. E. Tourtellotte, J. Bacterial., 1967, 93, 636.
PY - 1972
Y1 - 1972
N2 - 2H enriched lipids have been isolated from the plasma membranes of the organism Acholeplasma laidlawii B(PG9) grown on media supplemented with 97 atom% deuteriated lauric acid; g.c.-m.s. of lipid derived methyl esters are interpreted as indicating chain elongation of the deuteriated lauric acid by 1 or 2 protonated C2-units.
AB - 2H enriched lipids have been isolated from the plasma membranes of the organism Acholeplasma laidlawii B(PG9) grown on media supplemented with 97 atom% deuteriated lauric acid; g.c.-m.s. of lipid derived methyl esters are interpreted as indicating chain elongation of the deuteriated lauric acid by 1 or 2 protonated C2-units.
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U2 - 10.1039/C3972000719b
DO - 10.1039/C3972000719b
M3 - Article
AN - SCOPUS:0037604436
SN - 0022-4936
VL - 0
SP - 719b-720
JO - Journal of the Chemical Society, Chemical Communications
JF - Journal of the Chemical Society, Chemical Communications
IS - 12
ER -