Gas chromatographic method for determining strychnine residues in alfalfa

G. Miller, J. Warren, K. Gohre, L. Hanks

Research output: Contribution to journalArticlepeer-review

Abstract

A gas-liquid chromatographic procedure is described for determining strychnine in alfalfa with a nitrogen-phosphorus detector. Alfalfa samples containing strychnine residues are chopped and extracted in a blender using ethyl acetate, sodium carbonate, and sodium sulfate, and the organic extract is extracted with 0.1N H2SO4 solution, made basic and subsequently extracted with methylene chloride. Following concentration, the strychnine residues are quantitated by peak height, using an external standard. Recoveries for 0.05 ppm spikes were 88 ± 11%.

Original languageEnglish (US)
Pages (from-to)901-903
Number of pages3
JournalJournal of the Association of Official Analytical Chemists
Volume65
Issue number4
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)

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