Gamma-conglutin peptides from Andean lupin legume (Lupinus mutabilis Sweet) enhanced glucose uptake and reduced gluconeogenesis in vitro

Erika B. Muñoz; Diego A. Luna-Vital; Marco Fornasini; Manuel E. Baldeón; Elvira Gonzalez de Mejia

doi:10.1016/j.jff.2018.04.021

Gamma-conglutin peptides from Andean lupin legume (Lupinus mutabilis Sweet) enhanced glucose uptake and reduced gluconeogenesis in vitro

Erika B. Muñoz, Diego A. Luna-Vital, Marco Fornasini, Manuel E. Baldeón, Elvira Gonzalez de Mejia

Research output: Contribution to journal › Article › peer-review

Abstract

The present study aimed to analyze the mechanism of action of a hydrolyzed gamma-conglutin fraction (Cgh) extracted from Andean L. mutabilis legume on glucose metabolism. At 5 mg/mL, Cgh from L. mutabilis inhibited dipeptidyl peptidase IV activity (DPP-IV) (100%), and increased (p < 0.05) 6.5-fold the glucose uptake in a dual-layered enterocyte/adipocyte culture system compared to untreated cells, through an enhancement of glucose transporter type-4 translocation. In this model, insulin-induced glucose uptake was potentiated by addition of L. mutabilis hydrolysates. Also, Cgh L. mutabilis hydrolysates decreased (p < 0.05) by 50% gluconeogenesis in a dual-layered enterocyte/hepatocyte system and reduced phosphoenolpyruvate carboxykinase expression. These data showed that L. mutabilis hydrolysates can affect glucose metabolism inhibiting DPP-IV enzymatic activity, improving insulin receptor sensitivity and inhibiting hepatic gluconeogenesis.

Original language	English (US)
Pages (from-to)	339-347
Number of pages	9
Journal	Journal of Functional Foods
Volume	45
DOIs	https://doi.org/10.1016/j.jff.2018.04.021
State	Published - Jun 2018

Keywords

Diabetes
Gamma-conglutin
Glucose production
Glucose uptake
Lupinus mutabilis Sweet

ASJC Scopus subject areas

Food Science
Medicine (miscellaneous)
Nutrition and Dietetics

Online availability

10.1016/j.jff.2018.04.021

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title = "Gamma-conglutin peptides from Andean lupin legume (Lupinus mutabilis Sweet) enhanced glucose uptake and reduced gluconeogenesis in vitro",

abstract = "The present study aimed to analyze the mechanism of action of a hydrolyzed gamma-conglutin fraction (Cgh) extracted from Andean L. mutabilis legume on glucose metabolism. At 5 mg/mL, Cgh from L. mutabilis inhibited dipeptidyl peptidase IV activity (DPP-IV) (100%), and increased (p < 0.05) 6.5-fold the glucose uptake in a dual-layered enterocyte/adipocyte culture system compared to untreated cells, through an enhancement of glucose transporter type-4 translocation. In this model, insulin-induced glucose uptake was potentiated by addition of L. mutabilis hydrolysates. Also, Cgh L. mutabilis hydrolysates decreased (p < 0.05) by 50% gluconeogenesis in a dual-layered enterocyte/hepatocyte system and reduced phosphoenolpyruvate carboxykinase expression. These data showed that L. mutabilis hydrolysates can affect glucose metabolism inhibiting DPP-IV enzymatic activity, improving insulin receptor sensitivity and inhibiting hepatic gluconeogenesis.",

keywords = "Diabetes, Gamma-conglutin, Glucose production, Glucose uptake, Lupinus mutabilis Sweet",

author = "Mu{\~n}oz, {Erika B.} and Luna-Vital, {Diego A.} and Marco Fornasini and Balde{\'o}n, {Manuel E.} and {Gonzalez de Mejia}, Elvira",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

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doi = "10.1016/j.jff.2018.04.021",

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AU - Muñoz, Erika B.

AU - Luna-Vital, Diego A.

AU - Fornasini, Marco

AU - Baldeón, Manuel E.

AU - Gonzalez de Mejia, Elvira

PY - 2018/6

Y1 - 2018/6

N2 - The present study aimed to analyze the mechanism of action of a hydrolyzed gamma-conglutin fraction (Cgh) extracted from Andean L. mutabilis legume on glucose metabolism. At 5 mg/mL, Cgh from L. mutabilis inhibited dipeptidyl peptidase IV activity (DPP-IV) (100%), and increased (p < 0.05) 6.5-fold the glucose uptake in a dual-layered enterocyte/adipocyte culture system compared to untreated cells, through an enhancement of glucose transporter type-4 translocation. In this model, insulin-induced glucose uptake was potentiated by addition of L. mutabilis hydrolysates. Also, Cgh L. mutabilis hydrolysates decreased (p < 0.05) by 50% gluconeogenesis in a dual-layered enterocyte/hepatocyte system and reduced phosphoenolpyruvate carboxykinase expression. These data showed that L. mutabilis hydrolysates can affect glucose metabolism inhibiting DPP-IV enzymatic activity, improving insulin receptor sensitivity and inhibiting hepatic gluconeogenesis.

AB - The present study aimed to analyze the mechanism of action of a hydrolyzed gamma-conglutin fraction (Cgh) extracted from Andean L. mutabilis legume on glucose metabolism. At 5 mg/mL, Cgh from L. mutabilis inhibited dipeptidyl peptidase IV activity (DPP-IV) (100%), and increased (p < 0.05) 6.5-fold the glucose uptake in a dual-layered enterocyte/adipocyte culture system compared to untreated cells, through an enhancement of glucose transporter type-4 translocation. In this model, insulin-induced glucose uptake was potentiated by addition of L. mutabilis hydrolysates. Also, Cgh L. mutabilis hydrolysates decreased (p < 0.05) by 50% gluconeogenesis in a dual-layered enterocyte/hepatocyte system and reduced phosphoenolpyruvate carboxykinase expression. These data showed that L. mutabilis hydrolysates can affect glucose metabolism inhibiting DPP-IV enzymatic activity, improving insulin receptor sensitivity and inhibiting hepatic gluconeogenesis.

KW - Diabetes

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KW - Glucose production

KW - Glucose uptake

KW - Lupinus mutabilis Sweet

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Gamma-conglutin peptides from Andean lupin legume (Lupinus mutabilis Sweet) enhanced glucose uptake and reduced gluconeogenesis in vitro

Abstract

Keywords

ASJC Scopus subject areas

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