Fusobacteria as a Marker to Estimate the Abundance of Asian Carp and Total Fish Population In Illinois River

Wen-Tso Liu, Lin Ye, Camila Carlos, Ya Zhang, Takashi Narihiro, Masaru Nobu, Andrew F. Casper, Jon Amberg, Mark Gaikowski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To develop a genetic-based method for Asian carp and total fish surveillance, this study characterized the bacterial community in the guts of 129 fish (17 fish species in total) caught from different water bodies in the U.S. using 16S rRNA gene sequence as the biomarker. By comparing with the microbiota in other animal guts, including human, beef cattle, chicken, goose, swine, and dairy cattle, it was found that the phylum Fusobacteria is almost unique to freshwater fish. Further analysis showed that the majority of the Fusobacteria (> 90% for most fish) in fish guts mainly affiliated two clusters under the genera of Cetobacterium and Hados.Sed.Eubac.3, respectively. In addition, a Leptotrichia-related and a Cetobactrium-related cluster were found to be unique to bigmouth buffalo and silver carp, respectively. Based on these findings and the fact that Fusobacteria members are obligate anaerobic bacteria and cannot grow in river water, we have designed and developed a series of PCR primers for a microbial source tracking method, and are currently validating the specificity of these primers with known samples. In addition, we will apply these primers to estimate and correlate the abundance of Asian carp and total fish in water bodies with samples from different location along Illinois River.
Original languageEnglish (US)
Title of host publicationGreat Waters, Great Lands, Great Responsibilities: 76th Midwest Fish & Wildlife Conference, January 24-27, 2016, Grand Rapids Michigan
StatePublished - 2016

Keywords

  • INHS

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