Functionalized biomicroelectromechanical systems sensors for force response study at local adhesion sites of single living cells on substrates

M. Taher A. Saif, Chad Randall Sager, Sean Coyer

Research output: Contribution to journalArticle

Abstract

We present a method of measuring force response of a single living cell, attached to a substrate, in situ, by a functionalized microelectromechanical systems sensor that applies local deformation on the cell. The sensor is a single crystal silicon microcantilever beam with prescribed shape and geometry, and is coated by a thin layer of fibronectin. It is brought in contact with a cell to form adhesion cites, and is then moved by a piezoactuator to deform the cell locally. The force is transmitted from the adhesion site(s) on the cantilever to the sites on the substrate through the cytoskeleton. The interaction force between the cell and the cantilever is measured from the deformation of the cantilever and its spring constant, which can be obtained by several independent means. The force and the cell deformation can be 10 s of nano-Newtons and micrometers, respectively. We demonstrate the method using two families of force sensors with spring constants of 18 and 0.4 nN/μm. Several cells, endothelial and fibroblast, are deformed by tens of micrometers until the adhesion sites failed. Their force-deformation response shows strong linearity. Several possible mechanisms are discussed to explain the linear response.

Original languageEnglish (US)
Pages (from-to)950-961
Number of pages12
JournalAnnals of Biomedical Engineering
Volume31
Issue number8
DOIs
StatePublished - Sep 11 2003

Fingerprint

Adhesion
Cells
Sensors
Substrates
Endothelial cells
Fibroblasts
MEMS
Single crystals
Silicon
Geometry

Keywords

  • Buckling
  • Cytoskeleton
  • Endothelial cell
  • Fibroblast cell
  • Fibromectin
  • MEMS
  • Microcantilever
  • Truss

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Functionalized biomicroelectromechanical systems sensors for force response study at local adhesion sites of single living cells on substrates. / Saif, M. Taher A.; Sager, Chad Randall; Coyer, Sean.

In: Annals of Biomedical Engineering, Vol. 31, No. 8, 11.09.2003, p. 950-961.

Research output: Contribution to journalArticle

@article{bf5a4ba37eb64742b2d387e0c6fca8fc,
title = "Functionalized biomicroelectromechanical systems sensors for force response study at local adhesion sites of single living cells on substrates",
abstract = "We present a method of measuring force response of a single living cell, attached to a substrate, in situ, by a functionalized microelectromechanical systems sensor that applies local deformation on the cell. The sensor is a single crystal silicon microcantilever beam with prescribed shape and geometry, and is coated by a thin layer of fibronectin. It is brought in contact with a cell to form adhesion cites, and is then moved by a piezoactuator to deform the cell locally. The force is transmitted from the adhesion site(s) on the cantilever to the sites on the substrate through the cytoskeleton. The interaction force between the cell and the cantilever is measured from the deformation of the cantilever and its spring constant, which can be obtained by several independent means. The force and the cell deformation can be 10 s of nano-Newtons and micrometers, respectively. We demonstrate the method using two families of force sensors with spring constants of 18 and 0.4 nN/μm. Several cells, endothelial and fibroblast, are deformed by tens of micrometers until the adhesion sites failed. Their force-deformation response shows strong linearity. Several possible mechanisms are discussed to explain the linear response.",
keywords = "Buckling, Cytoskeleton, Endothelial cell, Fibroblast cell, Fibromectin, MEMS, Microcantilever, Truss",
author = "Saif, {M. Taher A.} and Sager, {Chad Randall} and Sean Coyer",
year = "2003",
month = "9",
day = "11",
doi = "10.1114/1.1591189",
language = "English (US)",
volume = "31",
pages = "950--961",
journal = "Annals of Biomedical Engineering",
issn = "0090-6964",
publisher = "Springer Netherlands",
number = "8",

}

TY - JOUR

T1 - Functionalized biomicroelectromechanical systems sensors for force response study at local adhesion sites of single living cells on substrates

AU - Saif, M. Taher A.

AU - Sager, Chad Randall

AU - Coyer, Sean

PY - 2003/9/11

Y1 - 2003/9/11

N2 - We present a method of measuring force response of a single living cell, attached to a substrate, in situ, by a functionalized microelectromechanical systems sensor that applies local deformation on the cell. The sensor is a single crystal silicon microcantilever beam with prescribed shape and geometry, and is coated by a thin layer of fibronectin. It is brought in contact with a cell to form adhesion cites, and is then moved by a piezoactuator to deform the cell locally. The force is transmitted from the adhesion site(s) on the cantilever to the sites on the substrate through the cytoskeleton. The interaction force between the cell and the cantilever is measured from the deformation of the cantilever and its spring constant, which can be obtained by several independent means. The force and the cell deformation can be 10 s of nano-Newtons and micrometers, respectively. We demonstrate the method using two families of force sensors with spring constants of 18 and 0.4 nN/μm. Several cells, endothelial and fibroblast, are deformed by tens of micrometers until the adhesion sites failed. Their force-deformation response shows strong linearity. Several possible mechanisms are discussed to explain the linear response.

AB - We present a method of measuring force response of a single living cell, attached to a substrate, in situ, by a functionalized microelectromechanical systems sensor that applies local deformation on the cell. The sensor is a single crystal silicon microcantilever beam with prescribed shape and geometry, and is coated by a thin layer of fibronectin. It is brought in contact with a cell to form adhesion cites, and is then moved by a piezoactuator to deform the cell locally. The force is transmitted from the adhesion site(s) on the cantilever to the sites on the substrate through the cytoskeleton. The interaction force between the cell and the cantilever is measured from the deformation of the cantilever and its spring constant, which can be obtained by several independent means. The force and the cell deformation can be 10 s of nano-Newtons and micrometers, respectively. We demonstrate the method using two families of force sensors with spring constants of 18 and 0.4 nN/μm. Several cells, endothelial and fibroblast, are deformed by tens of micrometers until the adhesion sites failed. Their force-deformation response shows strong linearity. Several possible mechanisms are discussed to explain the linear response.

KW - Buckling

KW - Cytoskeleton

KW - Endothelial cell

KW - Fibroblast cell

KW - Fibromectin

KW - MEMS

KW - Microcantilever

KW - Truss

UR - http://www.scopus.com/inward/record.url?scp=0042377561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042377561&partnerID=8YFLogxK

U2 - 10.1114/1.1591189

DO - 10.1114/1.1591189

M3 - Article

C2 - 12918910

AN - SCOPUS:0042377561

VL - 31

SP - 950

EP - 961

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

SN - 0090-6964

IS - 8

ER -