Functional significance of nuclear estrogen receptor subtypes in the liver of goldfish

Erik R. Nelson, Hamid R. Habibi

Research output: Contribution to journalArticle

Abstract

Estrogens work by binding to and activating specific estrogen receptors (ERs). Although mammals have two major nuclear ER subtypes (ERαand ERβ), three subtypes have been shown in teleost fish (ERα, ERβ-I, and ERβ-II). 17β-Estradiol stimulates the production of an egg yolk precursor protein (vitellogenin) in the liver of oviparous species, including the goldfish. However, the functional involvement of the ER subtypes in this process is not fully understood. Here, using primary goldfish hepatocytes, we test the hypothesis that all three ER subtypes are functionally involved in the liver of goldfish by using RNA interference to specifically knock-down the different ER subtypes. The results suggest that ERα is induced by estradiol through activation of the ERβ subtypes. This induction serves to sensitize the liver to further stimulation by estradiol. The knock-down results were supported by use of ER subtype specific antagonists. Sensitization by up-regulation of ERα is likely to be important for seasonal spawners such as goldfish, to bring about a change from somatic growth to reproductive development, and vitellogenesis. The novel data presented in this study provide strong support for the hypothesis that the goldfish ER subtypes play functional roles in the regulation of vitellogenin and ERα and provide a framework for the better understanding of ER signaling in fish and other vertebrates.

Original languageEnglish (US)
Pages (from-to)1668-1676
Number of pages9
JournalEndocrinology
Volume151
Issue number4
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

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Goldfish
Estrogen Receptors
Liver
Estradiol
Fishes
Oviparity
Vitellogenesis
Vitellogenins
Egg Proteins
RNA Interference

ASJC Scopus subject areas

  • Endocrinology

Cite this

Functional significance of nuclear estrogen receptor subtypes in the liver of goldfish. / Nelson, Erik R.; Habibi, Hamid R.

In: Endocrinology, Vol. 151, No. 4, 01.04.2010, p. 1668-1676.

Research output: Contribution to journalArticle

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