Functional expression of RuBisCO reduces CO2 emission during fermentation by engineered Saccharomyces cerevisiae

Sujeong Park, Bo Ram Park, Deokyeol Jeong, Jongbeom Park, Ja Kyong Ko, Soo Jung Kim, Jeong Sun Kim, Yong Su Jin, Soo Rin Kim

Research output: Contribution to journalArticlepeer-review


Industrial biotechnology based on yeast fermentation is a promising strategy that can alleviate global warming and climate change. However, Saccharomyces cerevisiae, widely used in bioprocesses, releases a large amount of carbon dioxide (CO2) during fermentation. This study developed a mixotrophic CO2-fixing S. cerevisiae to achieve carbon neutrality and sustainability in bioprocess. A CO2-fixation pathway was constructed in a xylose-utilizing S. cerevisiae by heterologous expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) and phosphoribulokinase (PRK). Furthermore, a delta-integration strategy was utilized, and the RuBisCO gene copy number was increased to 10 copies to improve the efficiency of CO2-fixation. An additional Cas9-based genome editing was performed to overexpress other CO2-fixation related genes. The resulting CO2-fixing yeast, SJ03, exhibited the highest RuBisCO activity. During anaerobic xylose fermentation, ethanol concentration was increased by 17% and ethanol yield was increased by 16% compared to the control strain. In addition, CO2 emissions decreased by 7%. These results suggest that overexpression of the CO2-fixation pathway coupled with xylose utilization in S. cerevisiae might reduce CO2 emission in bioprocesses.

Original languageEnglish (US)
Pages (from-to)286-293
Number of pages8
JournalProcess Biochemistry
StatePublished - Nov 2023


  • 5-bisphosphate carboxylase/oxygenase
  • CRISPR/Cas9
  • Carbon dioxide fixation
  • Delta-integration strategy
  • Ribulose-1
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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