Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants

Suresh K. Mittal, Suresh K. Tikoo, J. V. Van Den Hurk, Michelle M. Breker-Klassen, Dongwan Yoo, Lorne A. Babiuk

Research output: Contribution to journalArticlepeer-review

Abstract

We constructed replication-competent human adenovirus type 5 (HAd5) recombinants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the control of the simian virus 40 (SV40) regulatory sequences. These genes were inserted in the early region 3 (E3) of the HAd5 genome in the E3 parallel orientation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extracts, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum. Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells.

Original languageEnglish (US)
Pages (from-to)253-260
Number of pages8
JournalIntervirology
Volume41
Issue number6
DOIs
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • Adenovirus recombinants
  • Adenovirus vectors
  • BPIV3 HN and F genes
  • Bovine parainfluenza virus type 3
  • Recombinant vaccine

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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