Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants

Suresh K. Mittal; Suresh K. Tikoo; J. V. Van Den Hurk; Michelle M. Breker-Klassen; Dongwan Yoo; Lorne A. Babiuk

doi:10.1159/000024947

Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants

Suresh K. Mittal, Suresh K. Tikoo, J. V. Van Den Hurk, Michelle M. Breker-Klassen, Dongwan Yoo, Lorne A. Babiuk

Research output: Contribution to journal › Article › peer-review

Abstract

We constructed replication-competent human adenovirus type 5 (HAd5) recombinants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the control of the simian virus 40 (SV40) regulatory sequences. These genes were inserted in the early region 3 (E3) of the HAd5 genome in the E3 parallel orientation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extracts, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum. Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells.

Original language	English (US)
Pages (from-to)	253-260
Number of pages	8
Journal	Intervirology
Volume	41
Issue number	6
DOIs	https://doi.org/10.1159/000024947
State	Published - 1998
Externally published	Yes

Keywords

Adenovirus recombinants
Adenovirus vectors
BPIV3 HN and F genes
Bovine parainfluenza virus type 3
Recombinant vaccine

ASJC Scopus subject areas

Virology
Infectious Diseases

Online availability

10.1159/000024947

Library availability

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title = "Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants",

abstract = "We constructed replication-competent human adenovirus type 5 (HAd5) recombinants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the control of the simian virus 40 (SV40) regulatory sequences. These genes were inserted in the early region 3 (E3) of the HAd5 genome in the E3 parallel orientation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extracts, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum. Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells.",

keywords = "Adenovirus recombinants, Adenovirus vectors, BPIV3 HN and F genes, Bovine parainfluenza virus type 3, Recombinant vaccine",

author = "Mittal, {Suresh K.} and Tikoo, {Suresh K.} and {Van Den Hurk}, {J. V.} and Breker-Klassen, {Michelle M.} and Dongwan Yoo and Babiuk, {Lorne A.}",

year = "1998",

doi = "10.1159/000024947",

language = "English (US)",

volume = "41",

pages = "253--260",

journal = "Intervirology",

issn = "0300-5526",

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T1 - Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants

AU - Mittal, Suresh K.

AU - Tikoo, Suresh K.

AU - Van Den Hurk, J. V.

AU - Breker-Klassen, Michelle M.

AU - Yoo, Dongwan

AU - Babiuk, Lorne A.

PY - 1998

Y1 - 1998

N2 - We constructed replication-competent human adenovirus type 5 (HAd5) recombinants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the control of the simian virus 40 (SV40) regulatory sequences. These genes were inserted in the early region 3 (E3) of the HAd5 genome in the E3 parallel orientation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extracts, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum. Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells.

AB - We constructed replication-competent human adenovirus type 5 (HAd5) recombinants (HAd5-HN and HAd5-F) containing the bovine parainfluenza virus type 3 (BPIV3) hemagglutinin-neuraminidase (HN) or fusion (F) gene under the control of the simian virus 40 (SV40) regulatory sequences. These genes were inserted in the early region 3 (E3) of the HAd5 genome in the E3 parallel orientation. Expression of HN or F in HAd5-HN- or HAd5-F-infected cell extracts, respectively, was observed by immunoprecipitation using a BPIV3-specific polyclonal antiserum. Our results suggest that HN and F expressed by HAd5 recombinants were functionally similar to the native HN and F expressed in BPIV3-infected cells.

KW - Adenovirus recombinants

KW - Adenovirus vectors

KW - BPIV3 HN and F genes

KW - Bovine parainfluenza virus type 3

KW - Recombinant vaccine

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U2 - 10.1159/000024947

DO - 10.1159/000024947

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SN - 0300-5526

VL - 41

SP - 253

EP - 260

JO - Intervirology

JF - Intervirology

IS - 6

ER -

Functional characterization of bovine parainfluenza virus type 3 hemagglutinin-neuraminidase and fusion proteins expressed by adenovirus recombinants

Abstract

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