Fumonisin B1 increases serum sphinganine concentration but does not alter serum sphingosine concentration or induce cardiovascular changes in milk-fed calves

Sheerin Mathur, Peter D. Constable, Robert M. Eppley, Mike E. Tumbleson, Geoffrey W. Smith, William J. Tranquilli, Dawn E. Morin, Wanda M. Haschek

Research output: Contribution to journalArticlepeer-review

Abstract

Fumonisin B1 is the most toxic and commonly occurring form of a group of mycotoxins that alter sphingolipid biosynthesis and induce leukoencephalomalacia in horses and pulmonary edema in pigs. Purified fumonisin B1 (1 mg/kg, iv, daily) increased serum sphinganine and sphingosine concentrations and decreased cardiovascular function in pigs within 5 days. We therefore examined whether the same dosage schedule of fumonisin B1 produced a similar effect in calves. Ten milk-fed male Holstein calves were instrumented to obtain blood and cardiovascular measurements. Treated calves (n = 5) were administered purified fumonisin B1 at 1 mg/kg, iv, daily for 7 days and controls (n = 5) were administered 10 ml 0.9% NaCl, iv, daily. Each calf was euthanized on day 7. In treated calves, serum sphinganine concentration increased from day 3 onward (day 7, 0.237 ± 0.388 μmol/l; baseline, 0.010 ± 0.007 μmol/l; mean ± SD), whereas, serum sphingosine concentration was unchanged (day 7, 0.044 ± 0.065 μmol/l; baseline, 0.021 ± 0.025 μmol/l). Heart rate, cardiac output, stroke volume, mean arterial pressure, mean pulmonary artery pressure, pulmonary artery wedge pressure, central venous pressure, plasma volume, base-apex electrocardiogram, arterial Po2, and systemic oxygen delivery were unchanged in treated and control calves. Fumonisin-treated calves developed metabolic acidosis (arterial blood pH, 7.27 ± 0.11; base excess, -9.1 ± 7.6 mEq/l), but all survived for 7 days. We conclude that calves are more resistant to fumonisin B1 cardiovascular toxicity than pigs.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalToxicological Sciences
Volume60
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Cardiovascular toxicity
  • Fumonisin
  • Metabolic acidosis
  • Sphinganine
  • Sphingolipid
  • Sphingosine

ASJC Scopus subject areas

  • Toxicology

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