Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein

Go Woon Choi, June Pyo Oh, In Sook Cho, Hye Kyoung Ju, Wen Xing Hu, Boram Kim, Eun Young Seo, Jong Seok Park, Leslie L. Domier, John Hammond, Kihak Song, Hyoun Sub Lim

Research output: Contribution to journalComment/debate

Abstract

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

Original languageEnglish (US)
Pages (from-to)538-542
Number of pages5
JournalPlant Pathology Journal
Volume35
Issue number5
DOIs
StatePublished - Jan 1 2019

Keywords

  • Difference of infectivity
  • Full-length infectious cDNA clone
  • Tobamovirus
  • Tomato mosaic virus (ToMV)

ASJC Scopus subject areas

  • Agronomy and Crop Science

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