Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein

Go Woon Choi; June Pyo Oh; In Sook Cho; Hye Kyoung Ju; Wen Xing Hu; Boram Kim; Eun Young Seo; Jong Seok Park; Leslie L. Domier; John Hammond; Kihak Song; Hyoun Sub Lim

doi:10.5423/PPJ.NT.12.2018.0286

Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein

Go Woon Choi, June Pyo Oh, In Sook Cho, Hye Kyoung Ju, Wen Xing Hu, Boram Kim, Eun Young Seo, Jong Seok Park, Leslie L. Domier, John Hammond, Kihak Song, Hyoun Sub Lim

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

Original language	English (US)
Pages (from-to)	538-542
Number of pages	5
Journal	Plant Pathology Journal
Volume	35
Issue number	5
DOIs	https://doi.org/10.5423/PPJ.NT.12.2018.0286
State	Published - 2019

Keywords

Difference of infectivity
Full-length infectious cDNA clone
Tobamovirus
Tomato mosaic virus (ToMV)

ASJC Scopus subject areas

Agronomy and Crop Science

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10.5423/PPJ.NT.12.2018.0286

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Choi, G. W., Oh, J. P., Cho, I. S., Ju, H. K., Hu, W. X., Kim, B., Seo, E. Y., Park, J. S., Domier, L. L., Hammond, J., Song, K., & Lim, H. S. (2019). Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein. Plant Pathology Journal, 35(5), 538-542. https://doi.org/10.5423/PPJ.NT.12.2018.0286

Choi, GW, Oh, JP, Cho, IS, Ju, HK, Hu, WX, Kim, B, Seo, EY, Park, JS, Domier, LL, Hammond, J, Song, K & Lim, HS 2019, 'Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein', Plant Pathology Journal, vol. 35, no. 5, pp. 538-542. https://doi.org/10.5423/PPJ.NT.12.2018.0286

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title = "Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein",

abstract = "In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.",

keywords = "Difference of infectivity, Full-length infectious cDNA clone, Tobamovirus, Tomato mosaic virus (ToMV)",

author = "Choi, {Go Woon} and Oh, {June Pyo} and Cho, {In Sook} and Ju, {Hye Kyoung} and Hu, {Wen Xing} and Boram Kim and Seo, {Eun Young} and Park, {Jong Seok} and Domier, {Leslie L.} and John Hammond and Kihak Song and Lim, {Hyoun Sub}",

note = "Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Project No. PJ01365501), Rural Development Administration, Republic of Korea. Publisher Copyright: {\textcopyright} The Korean Society of Plant Pathology.",

year = "2019",

doi = "10.5423/PPJ.NT.12.2018.0286",

language = "English (US)",

volume = "35",

pages = "538--542",

journal = "Plant Pathology Journal",

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T1 - Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein

AU - Choi, Go Woon

AU - Oh, June Pyo

AU - Cho, In Sook

AU - Ju, Hye Kyoung

AU - Hu, Wen Xing

AU - Kim, Boram

AU - Seo, Eun Young

AU - Park, Jong Seok

AU - Domier, Leslie L.

AU - Hammond, John

AU - Song, Kihak

AU - Lim, Hyoun Sub

N1 - Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Project No. PJ01365501), Rural Development Administration, Republic of Korea. Publisher Copyright: © The Korean Society of Plant Pathology.

PY - 2019

Y1 - 2019

N2 - In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

AB - In 2017, two new tomato mosaic virus (ToMV) isolates were collected from greenhouses in Buyeo, Chungcheongnam-do, South Korea. Full-length cDNAs of the new ToMV isolates were cloned into dual cauliflower mosaic virus 35S and T7 promoter-driven vectors, sequenced and their pathogenicities investigated. The nucleotide sequences of isolates GW1 (MH507165) and GW2 (MH507166) were 99% identical, resulting in only two amino acid differences in nonconserved region II and the helicase domain, Ile668Thr and Val834Ile. The two isolates were most closely related to a ToMV isolate from Taiwan (KJ207374). Isolate GW1 (Ile668, Val834) induced a systemic hypersensitive response in Nicotiana benthamiana compared with the isolate GW2, which a single residue substitution showed was due to Val834.

KW - Difference of infectivity

KW - Full-length infectious cDNA clone

KW - Tobamovirus

KW - Tomato mosaic virus (ToMV)

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Full-length infectious clones of two new isolates of tomato mosaic virus induce distinct symptoms associated with two differential amino acid residues in 128-kDa protein

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