FTIR nanobiosensors for Escherichia coli detection

Stefania Mura, Gianfranco Greppi, Maria Laura Marongiu, Pier Paolo Roggero, Sandeep P. Ravindranath, Lisa J. Mauer, Nicoletta Schibeci, Francesco Perria, Massimo Piccinini, Plinio Innocenzi, Joseph Irudayaraj

Research output: Contribution to journalArticlepeer-review

Abstract

Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl) triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective method for the effective screening of water samples.

Original languageEnglish (US)
Pages (from-to)485-492
Number of pages8
JournalBeilstein Journal of Nanotechnology
Volume3
Issue number1
DOIs
StatePublished - 2012

Keywords

  • Biosensors
  • E. Coli
  • Foodborne Pathogens
  • Ftir Spectroscopy
  • Nanomaterials

ASJC Scopus subject areas

  • Materials Science(all)
  • Physics and Astronomy(all)
  • Electrical and Electronic Engineering

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