FTIR nanobiosensors for Escherichia coli detection

Stefania Mura; Gianfranco Greppi; Maria Laura Marongiu; Pier Paolo Roggero; Sandeep P. Ravindranath; Lisa J. Mauer; Nicoletta Schibeci; Francesco Perria; Massimo Piccinini; Plinio Innocenzi; Joseph Irudayaraj

doi:10.3762/bjnano.3.55

FTIR nanobiosensors for Escherichia coli detection

Stefania Mura, Gianfranco Greppi, Maria Laura Marongiu, Pier Paolo Roggero, Sandeep P. Ravindranath, Lisa J. Mauer, Nicoletta Schibeci, Francesco Perria, Massimo Piccinini, Plinio Innocenzi, Joseph Irudayaraj

Research output: Contribution to journal › Article › peer-review

Abstract

Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl) triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 10² CFU/mL, constituting a simple and selective method for the effective screening of water samples.

Original language	English (US)
Pages (from-to)	485-492
Number of pages	8
Journal	Beilstein Journal of Nanotechnology
Volume	3
Issue number	1
DOIs	https://doi.org/10.3762/bjnano.3.55
State	Published - 2012
Externally published	Yes

Keywords

Biosensors
E. Coli
Foodborne Pathogens
Ftir Spectroscopy
Nanomaterials

ASJC Scopus subject areas

Materials Science(all)
Physics and Astronomy(all)
Electrical and Electronic Engineering

Online availability

10.3762/bjnano.3.55

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title = "FTIR nanobiosensors for Escherichia coli detection",

abstract = "Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl) triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective method for the effective screening of water samples.",

keywords = "Biosensors, E. Coli, Foodborne Pathogens, Ftir Spectroscopy, Nanomaterials",

author = "Stefania Mura and Gianfranco Greppi and Marongiu, {Maria Laura} and Roggero, {Pier Paolo} and Ravindranath, {Sandeep P.} and Mauer, {Lisa J.} and Nicoletta Schibeci and Francesco Perria and Massimo Piccinini and Plinio Innocenzi and Joseph Irudayaraj",

year = "2012",

doi = "10.3762/bjnano.3.55",

language = "English (US)",

volume = "3",

pages = "485--492",

journal = "Beilstein Journal of Nanotechnology",

issn = "2190-4286",

publisher = "Beilstein-Institut Zur Forderung der Chemischen Wissenschaften",

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T1 - FTIR nanobiosensors for Escherichia coli detection

AU - Mura, Stefania

AU - Greppi, Gianfranco

AU - Marongiu, Maria Laura

AU - Roggero, Pier Paolo

AU - Ravindranath, Sandeep P.

AU - Mauer, Lisa J.

AU - Schibeci, Nicoletta

AU - Perria, Francesco

AU - Piccinini, Massimo

AU - Innocenzi, Plinio

AU - Irudayaraj, Joseph

PY - 2012

Y1 - 2012

N2 - Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl) triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective method for the effective screening of water samples.

AB - Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl) triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 102 CFU/mL, constituting a simple and selective method for the effective screening of water samples.

KW - Biosensors

KW - E. Coli

KW - Foodborne Pathogens

KW - Ftir Spectroscopy

KW - Nanomaterials

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JF - Beilstein Journal of Nanotechnology

IS - 1

ER -

FTIR nanobiosensors for Escherichia coli detection

Abstract

Keywords

ASJC Scopus subject areas

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