FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

Karolina Chrabaszcz, Kamila Kochan, Andrzej Fedorowicz, Agnieszka Jasztal, Elzbieta Buczek, Lisa S. Leslie, Rohit Bhargava, Kamilla Malek, Stefan Chlopicki, Katarzyna M. Marzec

Research output: Contribution to journalArticle

Abstract

The combination of FT-IR and Raman spectroscopies allowed the biochemical profiling of lungs in the early stage of pulmonary metastasis in the murine model of breast cancer. Histological staining was used as a reference. Raman spectroscopy was especially useful in the detection and semi-quantitative analysis of the vitamin A content in lung lipofibroblasts, whereas the IR technique provided semi-quantitative information on the contents of nucleic acids, carbohydrates including glycogen, and lipids as well as changes in the secondary structures of tissue proteins. Our spectroscopic results suggest that the early phase of metastasis in the lung is characterized by a decrease in the endogenous retinoid content in combination with a decrease in the content of glycogen and lipids.

Original languageEnglish (US)
Pages (from-to)2042-2050
Number of pages9
JournalAnalyst
Volume143
Issue number9
DOIs
StatePublished - May 7 2018

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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    Chrabaszcz, K., Kochan, K., Fedorowicz, A., Jasztal, A., Buczek, E., Leslie, L. S., Bhargava, R., Malek, K., Chlopicki, S., & Marzec, K. M. (2018). FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice. Analyst, 143(9), 2042-2050. https://doi.org/10.1039/c7an01883e