TY - JOUR
T1 - From climate change to molecular response
T2 - Redox proteomics of ozone-induced responses in soybean
AU - Galant, Ashley
AU - Koester, Robert P.
AU - Ainsworth, Elizabeth A.
AU - Hicks, Leslie M.
AU - Jez, Joseph M.
PY - 2012/4
Y1 - 2012/4
N2 - • Ozone (O 3) causes significant agricultural losses, with soybean (Glycine max) being highly sensitive to this oxidant. Here we assess the effect of elevated seasonal O 3 exposure on the total and redox proteomes of soybean. • To understand the molecular responses to O 3 exposure, soybean grown at the Soybean Free Air Concentration Enrichment facility under ambient (37ppb), moderate (58ppb), and high (116ppb) O 3 concentrations was examined by redox-sensitive thiol labeling, mass spectrometry, and targeted enzyme assays. • Proteomic analysis of soybean leaf tissue exposed to high O 3 concentrations reveals widespread changes. In the high-O 3 treatment leaf, 35 proteins increased up to fivefold in abundance, 22 proteins showed up to fivefold higher oxidation, and 22 proteins increased in both abundance and oxidation. These changes occurred in carbon metabolism, photosynthesis, amino acid synthesis, flavonoid and isoprenoid biosynthesis, signaling and homeostasis, and antioxidant pathways. • This study shows that seasonal O 3 exposure in soybean alters the abundance and oxidation state of redox-sensitive multiple proteins and that these changes reflect a combination of damage effects and adaptive responses that influence a wide range of metabolic processes, which in some cases may help mitigate oxidative stress.
AB - • Ozone (O 3) causes significant agricultural losses, with soybean (Glycine max) being highly sensitive to this oxidant. Here we assess the effect of elevated seasonal O 3 exposure on the total and redox proteomes of soybean. • To understand the molecular responses to O 3 exposure, soybean grown at the Soybean Free Air Concentration Enrichment facility under ambient (37ppb), moderate (58ppb), and high (116ppb) O 3 concentrations was examined by redox-sensitive thiol labeling, mass spectrometry, and targeted enzyme assays. • Proteomic analysis of soybean leaf tissue exposed to high O 3 concentrations reveals widespread changes. In the high-O 3 treatment leaf, 35 proteins increased up to fivefold in abundance, 22 proteins showed up to fivefold higher oxidation, and 22 proteins increased in both abundance and oxidation. These changes occurred in carbon metabolism, photosynthesis, amino acid synthesis, flavonoid and isoprenoid biosynthesis, signaling and homeostasis, and antioxidant pathways. • This study shows that seasonal O 3 exposure in soybean alters the abundance and oxidation state of redox-sensitive multiple proteins and that these changes reflect a combination of damage effects and adaptive responses that influence a wide range of metabolic processes, which in some cases may help mitigate oxidative stress.
KW - Glycine max (soybean)
KW - Metabolism
KW - Oxidative stress
KW - Ozone (O )
KW - Proteomics
KW - Redox biology
UR - http://www.scopus.com/inward/record.url?scp=84857451367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857451367&partnerID=8YFLogxK
U2 - 10.1111/j.1469-8137.2011.04037.x
DO - 10.1111/j.1469-8137.2011.04037.x
M3 - Article
C2 - 22272738
AN - SCOPUS:84857451367
SN - 0028-646X
VL - 194
SP - 220
EP - 229
JO - New Phytologist
JF - New Phytologist
IS - 1
ER -