From climate change to molecular response: Redox proteomics of ozone-induced responses in soybean

Ashley Galant, Robert P. Koester, Elizabeth Ainsworth, Leslie M. Hicks, Joseph M. Jez

Research output: Contribution to journalArticle

Abstract

• Ozone (O 3) causes significant agricultural losses, with soybean (Glycine max) being highly sensitive to this oxidant. Here we assess the effect of elevated seasonal O 3 exposure on the total and redox proteomes of soybean. • To understand the molecular responses to O 3 exposure, soybean grown at the Soybean Free Air Concentration Enrichment facility under ambient (37ppb), moderate (58ppb), and high (116ppb) O 3 concentrations was examined by redox-sensitive thiol labeling, mass spectrometry, and targeted enzyme assays. • Proteomic analysis of soybean leaf tissue exposed to high O 3 concentrations reveals widespread changes. In the high-O 3 treatment leaf, 35 proteins increased up to fivefold in abundance, 22 proteins showed up to fivefold higher oxidation, and 22 proteins increased in both abundance and oxidation. These changes occurred in carbon metabolism, photosynthesis, amino acid synthesis, flavonoid and isoprenoid biosynthesis, signaling and homeostasis, and antioxidant pathways. • This study shows that seasonal O 3 exposure in soybean alters the abundance and oxidation state of redox-sensitive multiple proteins and that these changes reflect a combination of damage effects and adaptive responses that influence a wide range of metabolic processes, which in some cases may help mitigate oxidative stress.

Original languageEnglish (US)
Pages (from-to)220-229
Number of pages10
JournalNew Phytologist
Volume194
Issue number1
DOIs
StatePublished - Apr 1 2012

Fingerprint

Climate Change
Ozone
Soybeans
ozone
Proteomics
proteomics
Oxidation-Reduction
climate change
soybeans
oxidation
proteins
free air carbon dioxide enrichment
Proteins
isoprenoids
proteome
thiols
oxidants
Terpenes
Photosynthesis
Enzyme Assays

Keywords

  • Glycine max (soybean)
  • Metabolism
  • Oxidative stress
  • Ozone (O )
  • Proteomics
  • Redox biology

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

From climate change to molecular response : Redox proteomics of ozone-induced responses in soybean. / Galant, Ashley; Koester, Robert P.; Ainsworth, Elizabeth; Hicks, Leslie M.; Jez, Joseph M.

In: New Phytologist, Vol. 194, No. 1, 01.04.2012, p. 220-229.

Research output: Contribution to journalArticle

Galant, Ashley ; Koester, Robert P. ; Ainsworth, Elizabeth ; Hicks, Leslie M. ; Jez, Joseph M. / From climate change to molecular response : Redox proteomics of ozone-induced responses in soybean. In: New Phytologist. 2012 ; Vol. 194, No. 1. pp. 220-229.
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