Abstract

Label-free imaging has re-emerged as a premier approach for imaging living cells and tissues within their natural environment. Label-free imaging provides a non-destructive, high throughput platform for understanding and defining the biophysical states of cells. We anticipate that the real-time spatially-resolved information about the biophysical states of cells will be useful for evaluating and quantifying the changes of these states in response to a variety of external interventions. Here we present a portable high-resolution microscopy system which combines five imaging channels, including four simultaneously excited multiphoton imaging channels (3-photon, 2-photon, third and second harmonic) and wide-field near-infrared imaging.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX
EditorsCaroline Boudoux, James W. Tunnell
PublisherSPIE
ISBN (Electronic)9781510647695
DOIs
StatePublished - 2022
EventAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX 2022 - San Francisco, United States
Duration: Jan 23 2022Jan 24 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11949
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XX 2022
Country/TerritoryUnited States
CitySan Francisco
Period1/23/221/24/22

Keywords

  • Microscopy
  • second harmonic generation
  • third harmonic generation
  • three-photon
  • two-photon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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