FOXA2 depletion leads to mucus hypersecretion in canine airways with respiratory diseases

Woosuk Choi, Alina X. Yang, Michelle A. Waltenburg, Shawn Choe, Madeline Steiner, Ahmed Radwan, Jingjun Lin, Carol W Maddox, Adam W. Stern, Richard Lincoln Fredrickson, Gee Lau

Research output: Contribution to journalArticle

Abstract

Because of exposure to environmental pollutants, infectious agents, and genetic predisposition, companion animals develop respiratory illnesses similar to those in humans. Older dogs of smaller breeds develop canine infectious respiratory disease, chronic bronchitis, and chronic obstructive pulmonary disease, with chronic lung infection, airway goblet cell hyperplasia and metaplasia, and mucus hypersecretion. Excessive mucus clogs airways, reduces gas exchanges, disables the mucociliary clearance, and reduces drug penetration. The Forkhead box protein A2 (FOXA2) is a key transcriptional regulator that maintains airway mucus homeostasis. Prior studies have shown that FOXA2 expression is frequently depleted in diseased human airways. Unfortunately, FOXA2 depletion has not been examined in dogs. Our current study indicated that both single bacterial infection by Pseudomonas aeruginosa and Bordetella bronchiseptica and polymicrobial infection by viral/bacterial pathogens depleted FOXA2 in canine airways, resulting in goblet cell hyperplasia and metaplasia and excessive mucus production. Furthermore, P. aeruginosa virulence factor pyocyanin activated the antagonistic STAT6 and epidermal growth factor receptor signalling pathways to inhibit FOXA2. Unravelling the mechanism of FOXA2 inactivation will hasten the development of non-antibiotic therapeutics to improve mucociliary clearance of pathogens in canine airway.

Original languageEnglish (US)
Article numbere12957
JournalCellular Microbiology
Volume21
Issue number1
DOIs
StatePublished - Jan 2019

Fingerprint

Mucus
Canidae
Mucociliary Clearance
Goblet Cells
Metaplasia
Hyperplasia
Hepatocyte Nuclear Factor 3-beta
Pyocyanine
Bordetella Infections
Bordetella bronchiseptica
Dogs
Environmental Pollutants
Chronic Bronchitis
Pets
Virulence Factors
Genetic Predisposition to Disease
Coinfection
Bacterial Infections
Epidermal Growth Factor Receptor
Pseudomonas aeruginosa

Keywords

  • EGFR
  • FOXA2
  • STAT6
  • canine respiratory diseases
  • goblet cell hyperplasia and metaplasia
  • mucus hypersecretion

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Choi, W., Yang, A. X., Waltenburg, M. A., Choe, S., Steiner, M., Radwan, A., ... Lau, G. (2019). FOXA2 depletion leads to mucus hypersecretion in canine airways with respiratory diseases. Cellular Microbiology, 21(1), [e12957]. https://doi.org/10.1111/cmi.12957

FOXA2 depletion leads to mucus hypersecretion in canine airways with respiratory diseases. / Choi, Woosuk; Yang, Alina X.; Waltenburg, Michelle A.; Choe, Shawn; Steiner, Madeline; Radwan, Ahmed; Lin, Jingjun; Maddox, Carol W; Stern, Adam W.; Fredrickson, Richard Lincoln; Lau, Gee.

In: Cellular Microbiology, Vol. 21, No. 1, e12957, 01.2019.

Research output: Contribution to journalArticle

Choi, W, Yang, AX, Waltenburg, MA, Choe, S, Steiner, M, Radwan, A, Lin, J, Maddox, CW, Stern, AW, Fredrickson, RL & Lau, G 2019, 'FOXA2 depletion leads to mucus hypersecretion in canine airways with respiratory diseases', Cellular Microbiology, vol. 21, no. 1, e12957. https://doi.org/10.1111/cmi.12957
Choi, Woosuk ; Yang, Alina X. ; Waltenburg, Michelle A. ; Choe, Shawn ; Steiner, Madeline ; Radwan, Ahmed ; Lin, Jingjun ; Maddox, Carol W ; Stern, Adam W. ; Fredrickson, Richard Lincoln ; Lau, Gee. / FOXA2 depletion leads to mucus hypersecretion in canine airways with respiratory diseases. In: Cellular Microbiology. 2019 ; Vol. 21, No. 1.
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