Abstract
We review the basic principles of light-tissue interaction and common methods of investigation. The mathematical framework for describing weakly scattering regime (the Born approximation) as well as the strong scattering regime (the diffusion equation) are described. Traditional techniques based on polarization, time-resolved, single and multiple scattering are reviewed. We then introduce Fourier transform light scattering (FTLS), which is a recent development from our own laboratory. FTLS is the spatial analogue of Fourier transform spectroscopy, in the sense that it provides angular scattering (spatial frequency) data from phase and amplitude measurements in the spatial (image) domain. We show that FTLS can be used as a diagnostic tool by translating the quantitative phase information into data of clinical relevance. Further, FTLS allows us to extract scattering parameters of the tissue from imaging unlabeled, thin tissue slices, using a relationship which we call the scattering-phase theorem. Using these measurements, FTLS can predict the outcome of many other experiments, including time resolved and enhanced backscattering experiments.
| Original language | English (US) |
|---|---|
| Title of host publication | Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition |
| Publisher | Springer New York |
| Pages | 259-290 |
| Number of pages | 32 |
| Volume | 1-2 |
| ISBN (Electronic) | 9781461451761 |
| ISBN (Print) | 9781461451754 |
| DOIs | |
| State | Published - Jan 1 2013 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Fourier transform light scattering of tissues. / Kim, Taewoo; Sridharan, Shamira; Popescu, Gabriel.
Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition. Vol. 1-2 Springer New York, 2013. p. 259-290.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Fourier transform light scattering of tissues
AU - Kim, Taewoo
AU - Sridharan, Shamira
AU - Popescu, Gabriel
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We review the basic principles of light-tissue interaction and common methods of investigation. The mathematical framework for describing weakly scattering regime (the Born approximation) as well as the strong scattering regime (the diffusion equation) are described. Traditional techniques based on polarization, time-resolved, single and multiple scattering are reviewed. We then introduce Fourier transform light scattering (FTLS), which is a recent development from our own laboratory. FTLS is the spatial analogue of Fourier transform spectroscopy, in the sense that it provides angular scattering (spatial frequency) data from phase and amplitude measurements in the spatial (image) domain. We show that FTLS can be used as a diagnostic tool by translating the quantitative phase information into data of clinical relevance. Further, FTLS allows us to extract scattering parameters of the tissue from imaging unlabeled, thin tissue slices, using a relationship which we call the scattering-phase theorem. Using these measurements, FTLS can predict the outcome of many other experiments, including time resolved and enhanced backscattering experiments.
AB - We review the basic principles of light-tissue interaction and common methods of investigation. The mathematical framework for describing weakly scattering regime (the Born approximation) as well as the strong scattering regime (the diffusion equation) are described. Traditional techniques based on polarization, time-resolved, single and multiple scattering are reviewed. We then introduce Fourier transform light scattering (FTLS), which is a recent development from our own laboratory. FTLS is the spatial analogue of Fourier transform spectroscopy, in the sense that it provides angular scattering (spatial frequency) data from phase and amplitude measurements in the spatial (image) domain. We show that FTLS can be used as a diagnostic tool by translating the quantitative phase information into data of clinical relevance. Further, FTLS allows us to extract scattering parameters of the tissue from imaging unlabeled, thin tissue slices, using a relationship which we call the scattering-phase theorem. Using these measurements, FTLS can predict the outcome of many other experiments, including time resolved and enhanced backscattering experiments.
UR - http://www.scopus.com/inward/record.url?scp=84897665782&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897665782&partnerID=8YFLogxK
U2 - 10.1007/978-1-4614-5176-1_7
DO - 10.1007/978-1-4614-5176-1_7
M3 - Chapter
AN - SCOPUS:84897665782
SN - 9781461451754
VL - 1-2
SP - 259
EP - 290
BT - Handbook of Coherent-Domain Optical Methods: Biomedical Diagnostics, Environmental Monitoring, and Materials Science:: Second Edition
PB - Springer New York
ER -