Fourier Transform Infrared (FT-IR) spectroscopy coupled with filtration and immunomagnetic separation for the detection of Escherichia coli O157:H7 in ground beef

R. Davis, Y. Burgula, J. Irudayaraj, B. L. Reuhs, L. J. Mauer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The majority of Escherichia coli O157:H7 outbreaks are associated with ground beef. To detect this pathogen, separation techniques were tested with E. coli O157:H7 in ground beef followed by FT-IR analyses. Ground beef samples were inoculated with various levels of live and heat treated E. coli O157:H7 cells and the bacteria were extracted by filtration or immunomagnetic separation (IMS). Spectra were collected and detection limits were established by discriminant analysis of the 1800-800 cm-1 region and comparison to standard plate counts. The detection limit for the Filtration-FT-IR and IMS-FT-IR assays was 105 CFU/g. Partial least squares model established significant linear relationships between plate counts and spectra [R ≥ 0.99]. Discriminant analysis and canonical variate analysis of the spectra differentiated live and heat treated cells of E. coli O157:H7. Validation experiments using ground beef inoculated with fewer cells (101- 102 CFU/g) reached the detection limit within a six hour incubation. A portable IR sensor was also used to detect E. coli O157:H7 in ground beef, and the detection limit was 107 CFU/g. The total time to detection for Filtration-FT-IR and IMS-FT-IR were one hour and 3.75 h, respectively which is faster than conventional plate count methods (48h) and conventional IMS methods (48h). The FT-IR methods developed are potentially rapid and simple protocols that could be further developed for the detection of different species of pathogenic bacteria in complex food systems.

Original languageEnglish (US)
Title of host publication2010 18th Biennial University/Government/Industry Micro-Nano Symposium, UGIM 2010 - Proceedings
DOIs
StatePublished - Aug 24 2010
Externally publishedYes
Event18th Biennial University/Government/Industry Micro-Nano Symposium, UGIM 2010 - West Lafayette, IN, United States
Duration: Jun 28 2010Jul 1 2010

Publication series

NameBiennial University/Government/Industry Microelectronics Symposium - Proceedings
ISSN (Print)0749-6877

Other

Other18th Biennial University/Government/Industry Micro-Nano Symposium, UGIM 2010
CountryUnited States
CityWest Lafayette, IN
Period6/28/107/1/10

Keywords

  • Dynabeads®
  • E. coli O157:H7
  • FT-IR
  • Ground beef
  • Pathogen detection

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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    Davis, R., Burgula, Y., Irudayaraj, J., Reuhs, B. L., & Mauer, L. J. (2010). Fourier Transform Infrared (FT-IR) spectroscopy coupled with filtration and immunomagnetic separation for the detection of Escherichia coli O157:H7 in ground beef. In 2010 18th Biennial University/Government/Industry Micro-Nano Symposium, UGIM 2010 - Proceedings [5508916] (Biennial University/Government/Industry Microelectronics Symposium - Proceedings). https://doi.org/10.1109/UGIM.2010.5508916