Forced dissociation of the strand dimer interface between C-cadherin ectodomains.

M. V. Bayas, K. Schulten, Deborah E Leckband

Research output: Contribution to journalArticle

Abstract

The force-induced dissociation of the strand dimer interface in C-cadherin has been studied using steered molecular dynamics simulations. The dissociation occurred, without domain unraveling, after the extraction of the conserved trypthophans (Trp2) from their respective hydrophobic pockets. The simulations revealed two stable positions for the Trp2 side chain inside the pocket. The most internal stable position involved a hydrogen bond between the ring Nepsilon of Trp2 and the backbone carbonyl of Glu90. In the second stable position, the aromatic ring is located at the pocket entrance. After extracting the two tryptophans from their pockets, the complex exists in an intermediate bound state that involves a close packing of the tryptophans with residues Asp1 and Asp27 from both domains. Dissociation occurred after this residue association was broken. Simulations carried out with a complex formed between W2A mutants showed that the mutant complex dissociates more easily than the wild type complex does. These results correlate closely with the role of the conserved tryptophans suggested previously by site directed mutagenesis.

Original languageEnglish (US)
Pages (from-to)101-111
Number of pages11
JournalMechanics & chemistry of biosystems : MCB
Volume1
Issue number2
StatePublished - Jun 2004

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Cadherins
Tryptophan
Dimers
Mutagenesis
Molecular Dynamics Simulation
Site-Directed Mutagenesis
Molecular dynamics
Hydrogen
Hydrogen bonds
Association reactions
Computer simulation

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Forced dissociation of the strand dimer interface between C-cadherin ectodomains. / Bayas, M. V.; Schulten, K.; Leckband, Deborah E.

In: Mechanics & chemistry of biosystems : MCB, Vol. 1, No. 2, 06.2004, p. 101-111.

Research output: Contribution to journalArticle

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