Force response of single living cells due to localized deformation

T. Saif, C. Sager, S. Coyer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a method for measuring the mechanical response of a single cell in-situ when local deformation is applied at an adhesion site(s) by a functionalized MEMS (Micro Electro Mechanical Systems) force sensor with pN - nN force resolution, and with force and displacement ranges of 100s of nNs and μms. The force sensor is a micro mechanical cantilever beam made of single crystal silicon (SCS), coated by a thin layer of Fibronectin, an extra cellular matrix (ECM) protein, to activate cell adhesion. The end of the beam is brought in contact with a cell to form the adhesion site(s). The cantilever is then moved away from the cell to locally deform it. The force on the cell is measured from the deformation of the cantilever until the adhesion sites fails. We demonstrate the method by deforming several endothelial and fibroblast cells. Force response of the fibroblast cell shows linear behavior.

Original languageEnglish (US)
Title of host publicationMicroelectromechanical Systems
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages591-593
Number of pages3
ISBN (Print)0791836428, 9780791836422
StatePublished - Jan 1 2002

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings

Fingerprint

Adhesion
Cells
Fibroblasts
Cell adhesion
Sensors
Cantilever beams
Single crystals
Proteins
Silicon

ASJC Scopus subject areas

  • Mechanical Engineering

Cite this

Saif, T., Sager, C., & Coyer, S. (2002). Force response of single living cells due to localized deformation. In Microelectromechanical Systems (pp. 591-593). (ASME International Mechanical Engineering Congress and Exposition, Proceedings). American Society of Mechanical Engineers (ASME).

Force response of single living cells due to localized deformation. / Saif, T.; Sager, C.; Coyer, S.

Microelectromechanical Systems. American Society of Mechanical Engineers (ASME), 2002. p. 591-593 (ASME International Mechanical Engineering Congress and Exposition, Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Saif, T, Sager, C & Coyer, S 2002, Force response of single living cells due to localized deformation. in Microelectromechanical Systems. ASME International Mechanical Engineering Congress and Exposition, Proceedings, American Society of Mechanical Engineers (ASME), pp. 591-593.
Saif T, Sager C, Coyer S. Force response of single living cells due to localized deformation. In Microelectromechanical Systems. American Society of Mechanical Engineers (ASME). 2002. p. 591-593. (ASME International Mechanical Engineering Congress and Exposition, Proceedings).
Saif, T. ; Sager, C. ; Coyer, S. / Force response of single living cells due to localized deformation. Microelectromechanical Systems. American Society of Mechanical Engineers (ASME), 2002. pp. 591-593 (ASME International Mechanical Engineering Congress and Exposition, Proceedings).
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