Folate receptors as potential therapeutic targets in choroid plexus tumors of SV40 transgenic mice

Todd A. Patrick, David M. Kranz, Terry A. Van Dyke, Edward J. Roy

Research output: Contribution to journalArticle

Abstract

A high affinity folate receptor is expressed in some human cancers, including choroid plexus tumors and ependymomas, and has been suggested as a target for therapeutics. In this report, the expression of folate receptors in an SV40 large T antigen transgenic mouse (SV11) was investigated. SV11 mice develop choroid plexus tumors, a property that may be related to the observation that SV40 has been isolated from human choroid plexus tumors and ependymomas. We report that SV11 choroid plexus tumors contain a high affinity folate receptor (K(D) of 1 nM), detectable by 125I-folate autoradiography and immunohistochemistry. Western blot analysis indicated an apparent molecular weight of 38 kDa. RT-PCR revealed the presence of transcripts for both alpha and beta isoforms of the folate receptor. Brain parenchyma has undetectable folate receptor, but normal choroid plexus has substantial levels (as does human choroid plexus). The folate receptors of the tumor are accessible from the bloodstream whereas those of the normal choroid plexus are not. Thus SV11 transgenic mice should be useful for evaluating therapeutic targeting of high affinity folate receptors, both for efficacy of specific agents and possible side effects.

Original languageEnglish (US)
Pages (from-to)111-123
Number of pages13
JournalJournal of Neuro-Oncology
Volume32
Issue number2
DOIs
StatePublished - Feb 25 1997

Fingerprint

Choroid Plexus Neoplasms
Folic Acid
Transgenic Mice
Choroid Plexus
Ependymoma
Therapeutics
Folate Receptor 2
Polyomavirus Transforming Antigens
Viral Tumor Antigens
Autoradiography
Neoplasms
Protein Isoforms
Molecular Weight
Western Blotting
Immunohistochemistry
Polymerase Chain Reaction

Keywords

  • Brain tumor
  • Choroid plexus carcinoma
  • Ependymoma
  • Folate binding protein
  • SV11 mice
  • SV40 T antigen

ASJC Scopus subject areas

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

Cite this

Folate receptors as potential therapeutic targets in choroid plexus tumors of SV40 transgenic mice. / Patrick, Todd A.; Kranz, David M.; Van Dyke, Terry A.; Roy, Edward J.

In: Journal of Neuro-Oncology, Vol. 32, No. 2, 25.02.1997, p. 111-123.

Research output: Contribution to journalArticle

@article{8d18df289a5248ab804467a54392c6cb,
title = "Folate receptors as potential therapeutic targets in choroid plexus tumors of SV40 transgenic mice",
abstract = "A high affinity folate receptor is expressed in some human cancers, including choroid plexus tumors and ependymomas, and has been suggested as a target for therapeutics. In this report, the expression of folate receptors in an SV40 large T antigen transgenic mouse (SV11) was investigated. SV11 mice develop choroid plexus tumors, a property that may be related to the observation that SV40 has been isolated from human choroid plexus tumors and ependymomas. We report that SV11 choroid plexus tumors contain a high affinity folate receptor (K(D) of 1 nM), detectable by 125I-folate autoradiography and immunohistochemistry. Western blot analysis indicated an apparent molecular weight of 38 kDa. RT-PCR revealed the presence of transcripts for both alpha and beta isoforms of the folate receptor. Brain parenchyma has undetectable folate receptor, but normal choroid plexus has substantial levels (as does human choroid plexus). The folate receptors of the tumor are accessible from the bloodstream whereas those of the normal choroid plexus are not. Thus SV11 transgenic mice should be useful for evaluating therapeutic targeting of high affinity folate receptors, both for efficacy of specific agents and possible side effects.",
keywords = "Brain tumor, Choroid plexus carcinoma, Ependymoma, Folate binding protein, SV11 mice, SV40 T antigen",
author = "Patrick, {Todd A.} and Kranz, {David M.} and {Van Dyke}, {Terry A.} and Roy, {Edward J.}",
year = "1997",
month = "2",
day = "25",
doi = "10.1023/A:1005713115147",
language = "English (US)",
volume = "32",
pages = "111--123",
journal = "Journal of Neuro-Oncology",
issn = "0167-594X",
publisher = "Kluwer Academic Publishers",
number = "2",

}

TY - JOUR

T1 - Folate receptors as potential therapeutic targets in choroid plexus tumors of SV40 transgenic mice

AU - Patrick, Todd A.

AU - Kranz, David M.

AU - Van Dyke, Terry A.

AU - Roy, Edward J.

PY - 1997/2/25

Y1 - 1997/2/25

N2 - A high affinity folate receptor is expressed in some human cancers, including choroid plexus tumors and ependymomas, and has been suggested as a target for therapeutics. In this report, the expression of folate receptors in an SV40 large T antigen transgenic mouse (SV11) was investigated. SV11 mice develop choroid plexus tumors, a property that may be related to the observation that SV40 has been isolated from human choroid plexus tumors and ependymomas. We report that SV11 choroid plexus tumors contain a high affinity folate receptor (K(D) of 1 nM), detectable by 125I-folate autoradiography and immunohistochemistry. Western blot analysis indicated an apparent molecular weight of 38 kDa. RT-PCR revealed the presence of transcripts for both alpha and beta isoforms of the folate receptor. Brain parenchyma has undetectable folate receptor, but normal choroid plexus has substantial levels (as does human choroid plexus). The folate receptors of the tumor are accessible from the bloodstream whereas those of the normal choroid plexus are not. Thus SV11 transgenic mice should be useful for evaluating therapeutic targeting of high affinity folate receptors, both for efficacy of specific agents and possible side effects.

AB - A high affinity folate receptor is expressed in some human cancers, including choroid plexus tumors and ependymomas, and has been suggested as a target for therapeutics. In this report, the expression of folate receptors in an SV40 large T antigen transgenic mouse (SV11) was investigated. SV11 mice develop choroid plexus tumors, a property that may be related to the observation that SV40 has been isolated from human choroid plexus tumors and ependymomas. We report that SV11 choroid plexus tumors contain a high affinity folate receptor (K(D) of 1 nM), detectable by 125I-folate autoradiography and immunohistochemistry. Western blot analysis indicated an apparent molecular weight of 38 kDa. RT-PCR revealed the presence of transcripts for both alpha and beta isoforms of the folate receptor. Brain parenchyma has undetectable folate receptor, but normal choroid plexus has substantial levels (as does human choroid plexus). The folate receptors of the tumor are accessible from the bloodstream whereas those of the normal choroid plexus are not. Thus SV11 transgenic mice should be useful for evaluating therapeutic targeting of high affinity folate receptors, both for efficacy of specific agents and possible side effects.

KW - Brain tumor

KW - Choroid plexus carcinoma

KW - Ependymoma

KW - Folate binding protein

KW - SV11 mice

KW - SV40 T antigen

UR - http://www.scopus.com/inward/record.url?scp=0031040856&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031040856&partnerID=8YFLogxK

U2 - 10.1023/A:1005713115147

DO - 10.1023/A:1005713115147

M3 - Article

C2 - 9120540

AN - SCOPUS:0031040856

VL - 32

SP - 111

EP - 123

JO - Journal of Neuro-Oncology

JF - Journal of Neuro-Oncology

SN - 0167-594X

IS - 2

ER -