Fluorescence spectroscopy of single molecule dynamics and its biological applications

Taekjip Ha, Alice Ting, Joy Liang, Jennifer Glass, Daniel Chemla, Pete Schultz, Shimon Weiss

Research output: Contribution to journalArticle

Abstract

The fluorescence spectroscopy of single molecule dynamics and its biological applications were studied. It provided tools for testing semi-classical and quantum mechanical theories of light-matter interaction and molecular interactions. Properties of a single fluorescent probe attached to a macromolecule such as very high sensitivity of the fluorophore to its immediate local environment, including the sensitivity to the presence of other fluorophores and quenchers near-by were studied. Its unique absorption and emission transition dipoles, which could be interrogated by polarized light, were also investigated for providing local structural information.

Original languageEnglish (US)
Pages (from-to)179-185
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3273
DOIs
StatePublished - Dec 1 1998

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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