Fluorescence imaging with one nanometer accuracy: Application to molecular motors

Ahmet Yildiz, Paul R. Selvin

Research output: Contribution to journalArticle

Abstract

We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within ∼1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion.

Original languageEnglish (US)
Pages (from-to)574-582
Number of pages9
JournalAccounts of chemical research
Volume38
Issue number7
DOIs
StatePublished - Jul 1 2005

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ASJC Scopus subject areas

  • Chemistry(all)

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