Fluorescence imaging with one nanometer accuracy: Application to molecular motors

Ahmet Yildiz, Paul R Selvin

Research output: Contribution to journalArticle

Abstract

We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within ∼1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion.

Original languageEnglish (US)
Pages (from-to)574-582
Number of pages9
JournalAccounts of chemical research
Volume38
Issue number7
DOIs
StatePublished - Jul 1 2005

Fingerprint

Myosin Type V
Fluorescence
Imaging techniques
Kinesin
Fluorophores
Optical transfer function
Coloring Agents
Lighting
myosin VI

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Fluorescence imaging with one nanometer accuracy : Application to molecular motors. / Yildiz, Ahmet; Selvin, Paul R.

In: Accounts of chemical research, Vol. 38, No. 7, 01.07.2005, p. 574-582.

Research output: Contribution to journalArticle

@article{a884b1f8108a47b4a24b3c6c60062cce,
title = "Fluorescence imaging with one nanometer accuracy: Application to molecular motors",
abstract = "We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within ∼1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion.",
author = "Ahmet Yildiz and Selvin, {Paul R}",
year = "2005",
month = "7",
day = "1",
doi = "10.1021/ar040136s",
language = "English (US)",
volume = "38",
pages = "574--582",
journal = "Accounts of Chemical Research",
issn = "0001-4842",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Fluorescence imaging with one nanometer accuracy

T2 - Application to molecular motors

AU - Yildiz, Ahmet

AU - Selvin, Paul R

PY - 2005/7/1

Y1 - 2005/7/1

N2 - We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within ∼1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion.

AB - We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within ∼1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion.

UR - http://www.scopus.com/inward/record.url?scp=23744499004&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23744499004&partnerID=8YFLogxK

U2 - 10.1021/ar040136s

DO - 10.1021/ar040136s

M3 - Article

C2 - 16028892

AN - SCOPUS:23744499004

VL - 38

SP - 574

EP - 582

JO - Accounts of Chemical Research

JF - Accounts of Chemical Research

SN - 0001-4842

IS - 7

ER -