Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration

J. V. Sweedler; J. B. Shear; H. A. Fishman; R. N. Zare; R. H. Scheller

doi:10.1021/ac00005a022

Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration

J. V. Sweedler, J. B. Shear, H. A. Fishman, R. N. Zare, R. H. Scheller

Research output: Contribution to journal › Article

Abstract

A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10^-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10^-20 mol range.

Original language	English (US)
Pages (from-to)	496-502
Number of pages	7
Journal	Analytical chemistry
Volume	63
Issue number	5
DOIs	https://doi.org/10.1021/ac00005a022
State	Published - Feb 15 1991
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1021/ac00005a022

Fingerprint Dive into the research topics of 'Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration'. Together they form a unique fingerprint.

Cite this

Sweedler, J. V., Shear, J. B., Fishman, H. A., Zare, R. N., & Scheller, R. H. (1991). Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration. Analytical chemistry, 63(5), 496-502. https://doi.org/10.1021/ac00005a022

@article{e0b696a3b1b948a79f4424d9743e2f06,

title = "Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration",

abstract = "A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10-20 mol range.",

author = "Sweedler, {J. V.} and Shear, {J. B.} and Fishman, {H. A.} and Zare, {R. N.} and Scheller, {R. H.}",

year = "1991",

month = feb,

day = "15",

doi = "10.1021/ac00005a022",

language = "English (US)",

volume = "63",

pages = "496--502",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "5",

}

TY - JOUR

T1 - Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration

AU - Sweedler, J. V.

AU - Shear, J. B.

AU - Fishman, H. A.

AU - Zare, R. N.

AU - Scheller, R. H.

PY - 1991/2/15

Y1 - 1991/2/15

N2 - A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10-20 mol range.

AB - A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10-20 mol range.

UR - http://www.scopus.com/inward/record.url?scp=0026129637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026129637&partnerID=8YFLogxK

U2 - 10.1021/ac00005a022

DO - 10.1021/ac00005a022

M3 - Article

C2 - 1905883

AN - SCOPUS:0026129637

VL - 63

SP - 496

EP - 502

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 5

ER -