Abstract
A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10-20 mol range.
Original language | English (US) |
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Pages (from-to) | 496-502 |
Number of pages | 7 |
Journal | Analytical Chemistry |
Volume | 63 |
Issue number | 5 |
DOIs | |
State | Published - Feb 15 1991 |
Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry