Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration

J. V. Sweedler; J. B. Shear; H. A. Fishman; R. N. Zare; R. H. Scheller

doi:10.1021/ac00005a022

Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration

J. V. Sweedler, J. B. Shear, H. A. Fishman, R. N. Zare, R. H. Scheller

Research output: Contribution to journal › Article › peer-review

Abstract

A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10^-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10^-20 mol range.

Original language	English (US)
Pages (from-to)	496-502
Number of pages	7
Journal	Analytical Chemistry
Volume	63
Issue number	5
DOIs	https://doi.org/10.1021/ac00005a022
State	Published - Feb 15 1991
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/ac00005a022

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title = "Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration",

abstract = "A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10-20 mol range.",

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T1 - Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration

AU - Sweedler, J. V.

AU - Shear, J. B.

AU - Fishman, H. A.

AU - Zare, R. N.

AU - Scheller, R. H.

PY - 1991/2/15

Y1 - 1991/2/15

N2 - A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10-20 mol range.

AB - A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of Images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluoresceln and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 × 10-20 mol; detection limits for FITC-amino acids are in the (2-8) × 10-20 mol range.

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Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration

Abstract

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