TY - GEN
T1 - Fluorescence correlation spectroscopy for detection of trace amount of biological agents
AU - Qing, De Kui
AU - Mengüç, M. Pinar
AU - Payne, Fred A.
AU - Danao, Mary Grace C.
PY - 2002
Y1 - 2002
N2 - A procedure to the trace amount of biological agents in water, based on fluorescence correlation spectroscopy and confocal microscopy, is explored. A special flow system is developed to increase the probability of detecting Escherichia coli (E. coli), and confocal microscopy is used to increase the sensitivity of the detection system. It is observed that concentrations higher than 1.5×105 E. coli per milliliter (2.5×10-16 M) are detectable with the present setup. This concentration corresponds to about 1.0 nM level of Rhodamine 6-G dyes. For detection of lower concentrations, further improvements need to be implemented to increase signal/background ratio. A detailed analysis of the optical system is presented and further improvements for the procedure are discussed.
AB - A procedure to the trace amount of biological agents in water, based on fluorescence correlation spectroscopy and confocal microscopy, is explored. A special flow system is developed to increase the probability of detecting Escherichia coli (E. coli), and confocal microscopy is used to increase the sensitivity of the detection system. It is observed that concentrations higher than 1.5×105 E. coli per milliliter (2.5×10-16 M) are detectable with the present setup. This concentration corresponds to about 1.0 nM level of Rhodamine 6-G dyes. For detection of lower concentrations, further improvements need to be implemented to increase signal/background ratio. A detailed analysis of the optical system is presented and further improvements for the procedure are discussed.
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U2 - 10.1115/IMECE2002-32357
DO - 10.1115/IMECE2002-32357
M3 - Conference contribution
AN - SCOPUS:78249241775
SN - 0791836509
SN - 9780791836507
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings
SP - 29
EP - 35
BT - Advances in Bioengineering
PB - American Society of Mechanical Engineers (ASME)
ER -