Fluorescence correlation spectroscopy for detection of trace amount of biological agents

De Kui Qing, M. Pinar Mengüç, Fred A. Payne, Mary-Grace Danao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A procedure to the trace amount of biological agents in water, based on fluorescence correlation spectroscopy and confocal microscopy, is explored. A special flow system is developed to increase the probability of detecting Escherichia coli (E. coli), and confocal microscopy is used to increase the sensitivity of the detection system. It is observed that concentrations higher than 1.5×105 E. coli per milliliter (2.5×10-16 M) are detectable with the present setup. This concentration corresponds to about 1.0 nM level of Rhodamine 6-G dyes. For detection of lower concentrations, further improvements need to be implemented to increase signal/background ratio. A detailed analysis of the optical system is presented and further improvements for the procedure are discussed.

Original languageEnglish (US)
Title of host publicationAdvances in Bioengineering
PublisherAmerican Society of Mechanical Engineers (ASME)
Pages29-35
Number of pages7
ISBN (Print)0791836509, 9780791836507
DOIs
StatePublished - Jan 1 2002

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings

ASJC Scopus subject areas

  • Mechanical Engineering

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