Fluorescence anisotropy microplate assay to investigate the interaction of full-length steroid receptor coactivator-1a with steroid receptors

Chen Zhang, Steven K. Nordeen, David J. Shapiro

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Estrogens, acting via estrogen receptor (ER) play key roles in growth, differentiation, and gene regulation in the reproductive, central nervous, and skeletal systems. ER-mediated gene transcription contributes to the development and spread of breast, uterine, and liver cancer. Steroid receptor coactivator-1a (SRC1a) belongs to the P160 family of coactivators, which is the best known of the many coactivators implicated in ER-mediated transactivation. Binding of full-length P160 coactivators to steroid receptors has been difficult to investigate in vitro. This chapter details how to investigate the interaction of SRC1a with ER using the fluorescence anisotropy/polarization microplate assay (FAMA).

Original languageEnglish (US)
Title of host publicationGene Regulation
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages339-351
Number of pages13
ISBN (Print)9781627032834
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume977
ISSN (Print)1064-3745

Keywords

  • Estrogen receptor
  • Fluorescence anisotropy
  • LxxLL motif
  • Microplate
  • Steroid receptor coactivator-1a

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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