Fluctuation correlation spectroscopy with a laser-scanning microscope: Exploiting the hidden time structure

Michelle A. Digman; Parijat Sengupta; Paul W. Wiseman; Claire M. Brown; Alan R. Horwitz; Enrico Gratton

doi:10.1529/biophysj.105.061788

Fluctuation correlation spectroscopy with a laser-scanning microscope: Exploiting the hidden time structure

Michelle A. Digman
, Parijat Sengupta
, Paul W. Wiseman
, Claire M. Brown
, Alan R. Horwitz
, Enrico Gratton

Bioengineering

Research output: Contribution to journal › Article › peer-review

Abstract

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

Original language	English (US)
Pages (from-to)	L33-L36
Journal	Biophysical journal
Volume	88
Issue number	5
DOIs	https://doi.org/10.1529/biophysj.105.061788
State	Published - May 2005

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Biophysics

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10.1529/biophysj.105.061788

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title = "Fluctuation correlation spectroscopy with a laser-scanning microscope: Exploiting the hidden time structure",

abstract = "Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.",

author = "Digman, \{Michelle A.\} and Parijat Sengupta and Wiseman, \{Paul W.\} and Brown, \{Claire M.\} and Horwitz, \{Alan R.\} and Enrico Gratton",

note = "Work supported in part by U54 GM064346 Cell Migration Consortium (M.D., A.H., C.B., and E.G.), the National Institutes of Health-P41 P41-RRO3155 (E.G. and P.S.), and the Natural Sciences and Engineering Research Council of Canada and the Canadian Institutes of Health Research (P.W.W.).",

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doi = "10.1529/biophysj.105.061788",

language = "English (US)",

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T2 - Exploiting the hidden time structure

AU - Digman, Michelle A.

AU - Sengupta, Parijat

AU - Wiseman, Paul W.

AU - Brown, Claire M.

AU - Horwitz, Alan R.

AU - Gratton, Enrico

N1 - Work supported in part by U54 GM064346 Cell Migration Consortium (M.D., A.H., C.B., and E.G.), the National Institutes of Health-P41 P41-RRO3155 (E.G. and P.S.), and the Natural Sciences and Engineering Research Council of Canada and the Canadian Institutes of Health Research (P.W.W.).

PY - 2005/5

Y1 - 2005/5

N2 - Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

AB - Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

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SP - L33-L36

JO - Biophysical journal

JF - Biophysical journal

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ER -

Fluctuation correlation spectroscopy with a laser-scanning microscope: Exploiting the hidden time structure

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