Fluctuation correlation spectroscopy with a laser-scanning microscope: Exploiting the hidden time structure

Michelle A. Digman, Parijat Sengupta, Paul W. Wiseman, Claire M. Brown, Alan R. Horwitz, Enrico Gratton

Research output: Contribution to journalArticlepeer-review

Abstract

Images obtained with a laser-scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser-scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second timescale.

Original languageEnglish (US)
Pages (from-to)L33-L36
JournalBiophysical journal
Volume88
Issue number5
DOIs
StatePublished - May 2005

ASJC Scopus subject areas

  • Biophysics

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