Flow cytometric DNA analysis of wheat‐rye addition lines

A. Bashir, J. A. Auger, A Lane Rayburn

Research output: Contribution to journalArticle

Abstract

The nuclear DNA content of the 7 wheat‐rye chromosome addition lines were determined by flow cytometry. The nuclear DNA content of each line was compared to the original wheat parental line. From this information, the amount of DNA per rye chromosome was calculated. The DNA per chromosome ranged from 0.8 picograms (pg) in chromosome 3R to 1.4 pg in chromosome 7R. Flow cytometry was proven to be a rapid and reliable technique for the evaluation of aneuploid plant populations. Flow cytometry has the potential to allow for more rapid screening of aneuploid and euploid cytological stocks than conventional cytogenetic techniques. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)843-847
Number of pages5
JournalCytometry
Volume14
Issue number8
DOIs
StatePublished - Nov 1993

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Keywords

  • Flow cytometry
  • aneuploidy
  • germplasm evaluation
  • propidium iodide

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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