flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP‐binding protein

Phillip B. Carpenter, David W. Hanlon, George W. Ordal

Research output: Contribution to journalArticle

Abstract

we describe the sequence and characterization of the Bacillus subtilis flhF gene. flhF encodes a basic polypeptide of 41 kDa that contains a putative GTP‐binding motif. The sequence of FlhF reveals a structural relationship to two Escherichia coli proteins, Ffh and FtsY, as well as to other members of the SRP54 family, in a domain presumed to bind GTP. flhF is located in a large operon consisting of chemotaxis and flagellar genes. Cells deficient in flhF are non‐motile. Through the use of anti‐flagellar antibodies we have established that flhF is a flagellar (fla) gene. Thus, flhF is a unique flagellar gene in that it encodes a GTP‐binding protein with similarities to members of the SRP54 family of proteins. These data suggest that flagellar biosynthesis in B. subtilis requires GTP.

Original languageEnglish (US)
Pages (from-to)2705-2713
Number of pages9
JournalMolecular Microbiology
Volume6
Issue number18
DOIs
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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