TY - JOUR
T1 - Flavin-based fluorescent proteins
T2 - Emerging paradigms in biological imaging
AU - Mukherjee, Arnab
AU - Schroeder, Charles M.
N1 - Funding Information:
This work was supported by a Packard Fellowship from the David and Lucile Packard Foundation for CMS and an FMC Fellowship and a Graduate Dissertation Fellowship for AM.
PY - 2015/2
Y1 - 2015/2
N2 - Flavin-based fluorescent proteins (FbFPs) are an emerging class of fluorescent reporters characterized by oxygen-independent fluorescence and a small size - key advantages compared to the green fluorescent protein (GFP). FbFPs are at a nascent stage of development. However, they have already been used as versatile reporters for studying anaerobic biosystems and viral assemblies. Recently, FbFPs with improved brightness and photostability have been engineered. In addition, several FbFPs show high degrees of thermal and pH stability. For these reasons, FbFPs hold strong promise to extend bioimaging to clinically and industrially significant systems that have been challenging to study using GFPs. In this review, we highlight recent developments in the FbFP toolbox and explore further improvements necessary to maximize the potential of FbFPs.
AB - Flavin-based fluorescent proteins (FbFPs) are an emerging class of fluorescent reporters characterized by oxygen-independent fluorescence and a small size - key advantages compared to the green fluorescent protein (GFP). FbFPs are at a nascent stage of development. However, they have already been used as versatile reporters for studying anaerobic biosystems and viral assemblies. Recently, FbFPs with improved brightness and photostability have been engineered. In addition, several FbFPs show high degrees of thermal and pH stability. For these reasons, FbFPs hold strong promise to extend bioimaging to clinically and industrially significant systems that have been challenging to study using GFPs. In this review, we highlight recent developments in the FbFP toolbox and explore further improvements necessary to maximize the potential of FbFPs.
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U2 - 10.1016/j.copbio.2014.07.010
DO - 10.1016/j.copbio.2014.07.010
M3 - Review article
C2 - 25151058
AN - SCOPUS:84906220820
SN - 0958-1669
VL - 31
SP - 16
EP - 23
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -