Flagella from F18+ Escherichia coli play a role in adhesion to pig epithelial cell lines

Qiangde Duan; Mingxu Zhou; Xiaofang Zhu; Yang Yang; Jun Zhu; Wenbin Bao; Shenglong Wu; Xiaosai Ruan; Weiping Zhang; Guoqiang Zhu

doi:10.1016/j.micpath.2012.09.010

Flagella from F18+ Escherichia coli play a role in adhesion to pig epithelial cell lines

Qiangde Duan, Mingxu Zhou, Xiaofang Zhu, Yang Yang, Jun Zhu, Wenbin Bao, Shenglong Wu, Xiaosai Ruan, Weiping Zhang, Guoqiang Zhu

Research output: Contribution to journal › Article › peer-review

Abstract

F18 fimbriae and toxins produced by F18 fimbriae-carrying Escherichia coli (E. coli) strains are known virulence factors responsible for post-weaning diarrhea (PWD) and edema disease (ED). In this study, we showed that fliC isogenic mutants constructed in two reference wild-type F18 fimbriae (F18+) E. coli were markedly impaired in adherence in vitro cell models (p < 0.05). Flagella purified from F18+ E. coli could directly bind to cultured piglet epithelial cells and block adherence of F18+ E. coli to cells when pre-incubated. In addition, the F18+ E. coli fliC deletion mutants up-regulated the expression of type I fimbriae produced by F18+ E. coli strains. These results demonstrated that expression of flagella is essential for the adherence of F18+ E. coli in vitro.

Original language	English (US)
Pages (from-to)	32-38
Number of pages	7
Journal	Microbial Pathogenesis
Volume	55
Issue number	1
DOIs	https://doi.org/10.1016/j.micpath.2012.09.010
State	Published - Feb 2013
Externally published	Yes

Keywords

Adherence
Adhesin
F18+ E. coli
Flagella

ASJC Scopus subject areas

Microbiology
Infectious Diseases

Online availability

10.1016/j.micpath.2012.09.010

Library availability

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title = "Flagella from F18+ Escherichia coli play a role in adhesion to pig epithelial cell lines",

abstract = "F18 fimbriae and toxins produced by F18 fimbriae-carrying Escherichia coli (E. coli) strains are known virulence factors responsible for post-weaning diarrhea (PWD) and edema disease (ED). In this study, we showed that fliC isogenic mutants constructed in two reference wild-type F18 fimbriae (F18+) E. coli were markedly impaired in adherence in vitro cell models (p < 0.05). Flagella purified from F18+ E. coli could directly bind to cultured piglet epithelial cells and block adherence of F18+ E. coli to cells when pre-incubated. In addition, the F18+ E. coli fliC deletion mutants up-regulated the expression of type I fimbriae produced by F18+ E. coli strains. These results demonstrated that expression of flagella is essential for the adherence of F18+ E. coli in vitro.",

keywords = "Adherence, Adhesin, F18+ E. coli, Flagella",

author = "Qiangde Duan and Mingxu Zhou and Xiaofang Zhu and Yang Yang and Jun Zhu and Wenbin Bao and Shenglong Wu and Xiaosai Ruan and Weiping Zhang and Guoqiang Zhu",

note = "Funding Information: This study was supported by grants from the Chinese National Science Foundation Grant ( No.31072136 , 31270171 and No.30771603 ), the Jiangsu High Education Key Basic Science Foundation ( 08KJA230002 ), and the Genetically Modified Organisms Technology Major Project of China ( 2009ZX08006-004B ), a project founded by the Priority Acdemic Program of Development Jiangsu High Education Institution , Program for ChangJiang Scholars and Innovative Research Team In university “PCSIRT” ( IRT0978 ).",

year = "2013",

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doi = "10.1016/j.micpath.2012.09.010",

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pages = "32--38",

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publisher = "Academic Press Inc.",

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AU - Duan, Qiangde

AU - Zhou, Mingxu

AU - Zhu, Xiaofang

AU - Yang, Yang

AU - Zhu, Jun

AU - Bao, Wenbin

AU - Wu, Shenglong

AU - Ruan, Xiaosai

AU - Zhang, Weiping

AU - Zhu, Guoqiang

N1 - Funding Information: This study was supported by grants from the Chinese National Science Foundation Grant ( No.31072136 , 31270171 and No.30771603 ), the Jiangsu High Education Key Basic Science Foundation ( 08KJA230002 ), and the Genetically Modified Organisms Technology Major Project of China ( 2009ZX08006-004B ), a project founded by the Priority Acdemic Program of Development Jiangsu High Education Institution , Program for ChangJiang Scholars and Innovative Research Team In university “PCSIRT” ( IRT0978 ).

PY - 2013/2

Y1 - 2013/2

N2 - F18 fimbriae and toxins produced by F18 fimbriae-carrying Escherichia coli (E. coli) strains are known virulence factors responsible for post-weaning diarrhea (PWD) and edema disease (ED). In this study, we showed that fliC isogenic mutants constructed in two reference wild-type F18 fimbriae (F18+) E. coli were markedly impaired in adherence in vitro cell models (p < 0.05). Flagella purified from F18+ E. coli could directly bind to cultured piglet epithelial cells and block adherence of F18+ E. coli to cells when pre-incubated. In addition, the F18+ E. coli fliC deletion mutants up-regulated the expression of type I fimbriae produced by F18+ E. coli strains. These results demonstrated that expression of flagella is essential for the adherence of F18+ E. coli in vitro.

AB - F18 fimbriae and toxins produced by F18 fimbriae-carrying Escherichia coli (E. coli) strains are known virulence factors responsible for post-weaning diarrhea (PWD) and edema disease (ED). In this study, we showed that fliC isogenic mutants constructed in two reference wild-type F18 fimbriae (F18+) E. coli were markedly impaired in adherence in vitro cell models (p < 0.05). Flagella purified from F18+ E. coli could directly bind to cultured piglet epithelial cells and block adherence of F18+ E. coli to cells when pre-incubated. In addition, the F18+ E. coli fliC deletion mutants up-regulated the expression of type I fimbriae produced by F18+ E. coli strains. These results demonstrated that expression of flagella is essential for the adherence of F18+ E. coli in vitro.

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Flagella from F18+ Escherichia coli play a role in adhesion to pig epithelial cell lines

Abstract

Keywords

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