Flagella from F18+ Escherichia coli play a role in adhesion to pig epithelial cell lines

Qiangde Duan, Mingxu Zhou, Xiaofang Zhu, Yang Yang, Jun Zhu, Wenbin Bao, Shenglong Wu, Xiaosai Ruan, Weiping Zhang, Guoqiang Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

F18 fimbriae and toxins produced by F18 fimbriae-carrying Escherichia coli (E. coli) strains are known virulence factors responsible for post-weaning diarrhea (PWD) and edema disease (ED). In this study, we showed that fliC isogenic mutants constructed in two reference wild-type F18 fimbriae (F18+) E. coli were markedly impaired in adherence in vitro cell models (p < 0.05). Flagella purified from F18+ E. coli could directly bind to cultured piglet epithelial cells and block adherence of F18+ E. coli to cells when pre-incubated. In addition, the F18+ E. coli fliC deletion mutants up-regulated the expression of type I fimbriae produced by F18+ E. coli strains. These results demonstrated that expression of flagella is essential for the adherence of F18+ E. coli in vitro.

Original languageEnglish (US)
Pages (from-to)32-38
Number of pages7
JournalMicrobial Pathogenesis
Volume55
Issue number1
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

Keywords

  • Adherence
  • Adhesin
  • F18+ E. coli
  • Flagella

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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