Five newly collected turnip mosaic virus (Tumv) isolates from jeju island, korea are closely related to previously reported korean tumv isolates but show distinctive symptom development

Wen Xing Hu, Byoung Jo Kim, Younghwan Kwak, Eun Young Seo, Jung Kyu Kim, Jae Yeong Han, Ik Hyun Kim, Yong Pyo Lim, In Sook Cho, Leslie L. Domier, John Hammond�, Hyoun Sub Lim

Research output: Contribution to journalArticle

Abstract

For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bac- teriophage T7 RNA polymerase promoters. Their full-length sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.

Original languageEnglish (US)
Pages (from-to)381-388
Number of pages8
JournalPlant Pathology Journal
Volume35
Issue number4
DOIs
StatePublished - 2019

Keywords

  • Hypersensitive response
  • Mild symptoms
  • Turnip mosaic virus

ASJC Scopus subject areas

  • Agronomy and Crop Science

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