Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus

Tracy L. Shay, Stéphane Berghmans, Karin Segers, Stacy Meyers, Jonathan E. Beever, James E. Womack, Michel Georges, Carole Charlier, Noelle E. Cockett

Research output: Contribution to journalArticlepeer-review

Abstract

The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. The OAR18q linkage map and human HSA14q transcript map were aligned by genotyping two bovine-hamster whole-genome radiation hybrid panels with the microsatellite markers, as well as with sequences corresponding to HSA14q genes. Using Type I loci mapping to the IDVGA30-OY3 interval as anchor points, we have constructed a 1.4-Mb bovine BAC contig containing the IDVGA30-OY3 interval. We demonstrate that the IDVGA30-OY3 interval spans approximately 770 kb and contains at least four genes: YY1, WARS, DLK1, and GTL2.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalMammalian Genome
Volume12
Issue number2
DOIs
StatePublished - Feb 22 2001

ASJC Scopus subject areas

  • Genetics

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