@article{b4a367a8e03945d4a43759829e2d3db0,
title = "Fin healing and regeneration in sturgeon",
abstract = "Pectoral fin healing in fin spines and rays were examined in juvenile Atlantic sturgeon Acipenser oxyrinchus oxyrinchus following three different sampling techniques (n = 8–9 fish per treatment): entire leading fin spine removed, a 1–2 cm portion removed near the point of articulation, or a 1–2 cm portion removed from a secondary fin ray. Also, to determine whether antibiotic treatment influences healing, an additional group of fish (n = 8) was not given an injection of an oxytetracycline (OTC)-based antibiotic following removal of the entire leading fin spine. Following fin sampling, fish from different treatments were mixed equally between three large (4,000 I) recirculating systems and fin-ray healing and mortality were monitored over a 12 month period. To assess healing, blood samples were collected at 4 months to measure immune system responses, radiographs were taken at 4, 8 and 12 months to assess the degree of calcification in regions of damaged fins and fins were analyzed histologically at 12 months. Fish grew from a mean weight of 1.8 to 3.2 kg during the experiment and survival was near 100% in all treatments, with only one fish dying of unknown causes. Leukocyte counts, an indication of health status and survival were similar among treatments and in groups with or without antibiotic injection. Radiographs revealed mineralization took longer in fish with the entire leading fin spine removed and was the slowest near the point of articulation, presumably due to the greater structural support for the pectoral fin at this location. Histological sampling indicated spines and rays had similar healing patterns. Following injury, an orderly matrix of collagen bundles and many evenly spaced scleroblasts were present, transitioning to Sharpey fibres, with concentric layers forming lamellar bone. Healing and mineralization were characterized as periosteal osteogenesis and included embedded osteocytes surrounded by an osteoid seam. Chondroid formation was apparent in a few fractures not associated with treatments. The duration of time for external wound healing and internal mineralization of spines and rays depended on the fin treatment, with the slowest healing observed in fish with the most tissue removed, the entire leading fin spine.",
keywords = "Acipenser oxyrinchus, calcification, fin regeneration, healing, leukocytes, sturgeon",
author = "Allen, {Peter J.} and Wes Baumgartner and Erin Brinkman and DeVries, {Robert J.} and Stewart, {Heather A.} and Aboagye, {Daniel L.} and Ramee, {Shane W.} and Ciaramella, {Michael A.} and Culpepper, {Charlie M.} and Lora Petrie-Hanson",
note = "Funding Information: We thank the National Marine Fisheries Service and Northern Gulf Institute (grant#: NA11OAR4320199), the Mississippi Agricultural and Forestry Experiment Station (MAFES) and the US Department of Agriculture (USDA) Agricultural Research Service (project #58-6066-5-042) and the USDA National Institute of Food and Agriculture, State (project#: 1005154) for funding and facility support; K. Ware and J. Henne, US Fish and Wildlife Service Bears Bluff National Fish Hatchery, Wadmalaw Island, SC, for providing juvenile Atlantic sturgeon; Z. Mitchell, A. Omer, M. Fondren and staff at the Mississippi State University (MSU) South Farm Aquaculture Facility for assistance with fish care and sampling; R. Butler, MSU College of Veterinary Medicine (CVM) for assistance with radiology; C. Hohn, MSU CVM for assistance with blood smears; H. Schramm, for use of PIT tag equipment; and two anonymous reviewers for helpful comments in the review of this manuscript. Funding Information: information US Department of Agriculture Agricultural Research Service, Grant/Award Number: 58-6066-5-042; Mississippi Agricultural and Forestry Experiment Station; US Department of Agriculture National Institute of Food and Agriculture, Grant/Award Number: 1005154; National Marine Fisheries Service and Northern Gulf Institute, Grant/Award Number: NA11OAR4320199We thank the National Marine Fisheries Service and Northern Gulf Institute (grant#: NA11OAR4320199), the Mississippi Agricultural and Forestry Experiment Station (MAFES) and the US Department of Agriculture (USDA) Agricultural Research Service (project #58-6066-5-042) and the USDA National Institute of Food and Agriculture, State (project#: 1005154) for funding and facility support; K. Ware and J. Henne, US Fish and Wildlife Service Bears Bluff National Fish Hatchery, Wadmalaw Island, SC, for providing juvenile Atlantic sturgeon; Z. Mitchell, A. Omer, M. Fondren and staff at the Mississippi State University (MSU) South Farm Aquaculture Facility for assistance with fish care and sampling; R. Butler, MSU College of Veterinary Medicine (CVM) for assistance with radiology; C. Hohn, MSU CVM for assistance with blood smears; H. Schramm, for use of PIT tag equipment; and two anonymous reviewers for helpful comments in the review of this manuscript. Publisher Copyright: {\textcopyright} 2018 The Fisheries Society of the British Isles",
year = "2018",
month = nov,
doi = "10.1111/jfb.13794",
language = "English (US)",
volume = "93",
pages = "917--930",
journal = "Journal of Fish Biology",
issn = "0022-1112",
publisher = "Wiley-Blackwell",
number = "5",
}