Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells

Liang Zhu, Qing Zhang, Hanhua Feng, Simon Ang, Fook Siong Chau, Wen Tso Liu

Research output: Contribution to journalArticlepeer-review

Abstract

A filter-based microfluidic device was combined with immunofluorescent labeling as a platform to rapidly detect microbial cells. The coin-sized device consisted of micro-chambers, micro-channels and filter weirs (gap = 1-2 μm), and was demonstrated to effectively trap and concentrate microbial cells (i.e., Cryptosporidium parvum and Giardia lamblia), which were larger in size than the weir gap. After sample injection, a staining solution containing fluorescently-labeled antibodies was continuously provided into the device (flow rate = 20 μl min-1) to flush the microbial cells toward the weirs and to accelerate the fluorescent labeling reaction. Using a staining solution that was 10 to 100 times more dilute than the recommended concentration used in a conventional glass method, those target cells with a fluorescent signal-to-noise ratio of 12 could be microscopically observed at single-cell level within 2 to 5 min prior to secondary washing.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalLab on a chip
Volume4
Issue number4
DOIs
StatePublished - Aug 2004
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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