Abstract
A filter-based microfluidic device was combined with immunofluorescent labeling as a platform to rapidly detect microbial cells. The coin-sized device consisted of micro-chambers, micro-channels and filter weirs (gap = 1-2 μm), and was demonstrated to effectively trap and concentrate microbial cells (i.e., Cryptosporidium parvum and Giardia lamblia), which were larger in size than the weir gap. After sample injection, a staining solution containing fluorescently-labeled antibodies was continuously provided into the device (flow rate = 20 μl min-1) to flush the microbial cells toward the weirs and to accelerate the fluorescent labeling reaction. Using a staining solution that was 10 to 100 times more dilute than the recommended concentration used in a conventional glass method, those target cells with a fluorescent signal-to-noise ratio of 12 could be microscopically observed at single-cell level within 2 to 5 min prior to secondary washing.
Original language | English (US) |
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Pages (from-to) | 337-341 |
Number of pages | 5 |
Journal | Lab on a chip |
Volume | 4 |
Issue number | 4 |
DOIs | |
State | Published - Aug 2004 |
Externally published | Yes |
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- General Chemistry
- Biomedical Engineering